Significance, Detection and Elimination of ... - Minerva Biolabs

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Fluorescence method • Simple, direct indicator for vital mycoplasma • Little operative expense, but very time consuming • Poor indicator for mycoplasma species with tendencies of extra cellular cell absorption via cytadherent proteins • Seminars and experience required • Eur. Ph.-listed evidence -30-
Culture method • Strict requirements for the culture medium and growth conditions (aerobic/anaerobic), generally requires non-standard adjustments for the individual species • Extremely long testing times of 1 to 4 weeks • Difficult analysis • Broth and disk possible • Advantage: only living mycoplasma is detected • Sensitivity: 1 CFU corresponds to average 30 GU M. argininii Source: Mycoplasma Experience Ltd. bar = 1 mm -31-
Page 1 and 2: Significance, Detection and Elimina
Page 3 and 4: Cross-contamination The contaminati
Page 5 and 6: Yeast unicellular fungi size can va
Page 7 and 8: Fungi Medium color turns yellow qui
Page 9 and 10: Contamination with Pestivirus • B
Page 11 and 12: The Usual Suspects • Contaminatio
Page 13 and 14: Bacteria Contamination - Examples
Page 15 and 16: Analysis using Onar ® EUB 1 kb DNA
Page 17 and 18: Mycoplasma • Found in1898 classif
Page 19 and 20: Mycoplasma (continued) • Parasite
Page 21 and 22: Mycoplasma in the Electron Microsco
Page 23 and 24: Effects on Cell Culture • Inhibit
Page 25 and 26: Frequency and Source of Mycoplasma
Page 27 and 28: Sources of Contamination (continued
Page 29: Instruction for Testing • Regulat
Page 33 and 34: Alternative Mycoplasma Diagnostic M
Page 35 and 36: Performance Sample DNA-Extraction P
Page 37 and 38: Preparation of the Venor ® GeM Kit
Page 39 and 40: Features of Venor ® GeM • Valida
Page 41 and 42: Frequency of Testing • New cell a
Page 43 and 44: Mycoplasma Elimination Methods •
Page 45 and 46: Effect of Mynox ® on Mycoplasma El

Significance, Detection and Elimination of ... - Minerva Biolabs

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