Significance, Detection and Elimination of ... - Minerva Biolabs

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Procedure for Contaminated Cell Cultures/ Virus • Isolate the culture immediately (incubator and if possible autoclave) • Immediately lock and test cryo- conserves • Isolate all cryo-conserved samples • Inform all possible receipts • Standard disinfection of the laboratory • Initiate immediately treatment with irreplaceable cells -42-
Mycoplasma Elimination Methods • Antibiotics average activity: Ciprofloxacin (Ciprobay, Ciproxin), Doxycyclin low activity: Plasmocin, Chloramphenicol, Clindamycin, Azithromycin, Clarithromycin, Tetracyclin, Tiamulin no activity: Penicillin, Streptomycin, Polymyxin, Vancomyin, Erythromycin (only active against some species), Cephalosporine, Sulfametaxol, G418 (Geneticin, Gentamycin-Analogon), Bacitracin • Complement Fixation • Co-Cultivation with Macrophages • Physical and chemical methods heat inactivation at 40-42 °C photo inactivation with Hoechst 33258/5-Bromuracil liquid extraction • Autoclave -43-
Page 1 and 2: Significance, Detection and Elimina
Page 3 and 4: Cross-contamination The contaminati
Page 5 and 6: Yeast unicellular fungi size can va
Page 7 and 8: Fungi Medium color turns yellow qui
Page 9 and 10: Contamination with Pestivirus • B
Page 11 and 12: The Usual Suspects • Contaminatio
Page 13 and 14: Bacteria Contamination - Examples
Page 15 and 16: Analysis using Onar ® EUB 1 kb DNA
Page 17 and 18: Mycoplasma • Found in1898 classif
Page 19 and 20: Mycoplasma (continued) • Parasite
Page 21 and 22: Mycoplasma in the Electron Microsco
Page 23 and 24: Effects on Cell Culture • Inhibit
Page 25 and 26: Frequency and Source of Mycoplasma
Page 27 and 28: Sources of Contamination (continued
Page 29 and 30: Instruction for Testing • Regulat
Page 31 and 32: Culture method • Strict requireme
Page 33 and 34: Alternative Mycoplasma Diagnostic M
Page 35 and 36: Performance Sample DNA-Extraction P
Page 37 and 38: Preparation of the Venor ® GeM Kit
Page 39 and 40: Features of Venor ® GeM • Valida
Page 41: Frequency of Testing • New cell a
Page 45 and 46: Effect of Mynox ® on Mycoplasma El

Significance, Detection and Elimination of ... - Minerva Biolabs

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