Significance, Detection and Elimination of ... - Minerva Biolabs
Significance, Detection and Elimination of ... - Minerva Biolabs
Significance, Detection and Elimination of ... - Minerva Biolabs
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Recommendations for the Improvement<br />
<strong>of</strong> Mycoplasma Contamination<br />
• Regularly <strong>and</strong> sensitive testing (monthly); weekly testing in cases<br />
<strong>of</strong> laboratory contamination<br />
• Operate free <strong>of</strong> st<strong>and</strong>ards antibiotics<br />
• Whenever possible: separate the work benches <strong>and</strong> incubators for<br />
h<strong>and</strong>ling contaminated <strong>and</strong> mycoplasma free materials<br />
• Never use contained cultures; reject immediately or treated<br />
• Disinfect working surfaces <strong>and</strong> h<strong>and</strong>s with alcoholic spray before<br />
<strong>and</strong> after working procedures, or with the change <strong>of</strong> the working<br />
material<br />
• Quarantine new cells <strong>of</strong> any origin <strong>and</strong> integrate into the<br />
laboratory only after testing negative<br />
• For larger loads <strong>of</strong> serum, incubate on indicator cells 3T3 or Vero<br />
over 4 passages before integration <strong>and</strong> use, <strong>and</strong> the test<br />
supernatants by means <strong>of</strong> PCR<br />
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