Golniški simpozij 2011 Zbornik povzetkov
Golniški simpozij 2011 Zbornik povzetkov
Golniški simpozij 2011 Zbornik povzetkov
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Use of multiplex PCR for detection of<br />
bacterial respiratory tract pathogens<br />
Dane Luænik, Judit Stokiå, Viktorija Tomiœ, Franc Øifrer<br />
University Clinic of Respiratory and Allergic Diseases, Golnik<br />
Background<br />
To investigate the usefulness of multiplex polymerase chain reaction (mPCR) to detect Mycoplasma<br />
pneumoniae, Legionella pneumophila, and Chlamydophila pneumoniae in patients with suspected<br />
atypical community-acquired pneumonia and to assess the prevalence of Streptococcus pneumoniae<br />
and Haemophilus influenzae detection.<br />
Methods<br />
We evaluated retrospectively the results of mPCR in 210 patients asigned to 3 groups: patients with<br />
suspected atypical pneumonia (n = 110), patients with asthma (n = 27), and patients with diseases<br />
other than infection (heart failure, malignancy, pulmonary embolism, allergic reactions) (n = 73) admitted<br />
to University Clinic of Respiratory and Allergic Diseases Golnik between January 2010 and January<br />
<strong>2011</strong>.<br />
Results<br />
An atypical pathogen was detected only in the 26 patients in suspected atypical pneumonia group<br />
(23.6%). L. pneumophila was detected in 4 patients (3.6%), M. pneumoniae in 21 (19%), and C.<br />
pneumoniae in 1 patient (0.9%). No pathogen could be detected by mPCR in 24 (21.8%), 2 (7.4%),<br />
and 49 (67%) patients in suspected atypical pneumonia group, asthma, and other diseases group,<br />
respectively. In patients with suspected atypical pneumonia S. pneumoniae and H. influenzae were<br />
detected 61.8% and 30% patients, respectively. In asthmatic patients S. pneumoniae and H. influenzae<br />
were detected in 81.5% and 51.8%, respectively. In patients with other diseases S. pneumoniae<br />
and H. influenzae were detected in 31.5% and 23%, respectively.<br />
Conclusions<br />
mPCR can increase the number of microbiological detection of atypical pathogens in patients with<br />
suspected community-acquired pneumonia. Clinical relevance of positive mPCR for S. pneumoniae<br />
and H. influenzae is questionable since it cannot distinguish between colonization and infection and<br />
thus, further studies are needed.<br />
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