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Golniški simpozij 2011 Zbornik povzetkov

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Methicillin-resistant Staphylococcus<br />

aureus and extended-spectrum b-<br />

lactamase producing<br />

Enterobacteriaceae in house pets<br />

Katja Øinkovec 1 , Dane Luænik 2 , Zlata Œop 3 , Viktorija Tomiœ 2<br />

1<br />

Department of Microbiology, Faculty for Biotechnology, University of Ljubljana<br />

2<br />

University Clinic of Respiratory and Allergic Diseases, Golnik<br />

3<br />

Veterinary Clinic, Lesce<br />

Background<br />

Resistant bacteria are a major public health concern. Methicillin-resistant Staphylococcus aureus<br />

(MRSA) is a major pathogen in human medicine and has become a recognized problem in farm animals<br />

(e.g. pigs). Pet owners as well as veterinary doctors and assistants can be colonized with<br />

MRSA and/or with entended-spectrum b-lactamase producing Enterobacteriaceae (ESBL-E) and<br />

they could transfer the pathogens to their pets through direct contact. We wanted to determine the<br />

carriage rate of both pathogens in house pets attending a busy veterinary clinic in Lesce (NW Slovenia).<br />

Methods<br />

A survey was conducted prospectively from February 1 to May 1, <strong>2011</strong> Veterinary Clinic Lesce.<br />

House pets, mainly cats and dogs were screened for carriage of ESBL-E and MRSA on a visit to the<br />

clinic. To assess carriage of MRSA nose and rectal swabs and for carriage of ESBL-E only rectal<br />

swabs were collected. All swabs were inoculated into brain-heart infusion broth. After 24 h of incubation,<br />

broths were subcultured onto BBL CHROMagar MRSA (BD, USA) and Brilliance ESBL agar<br />

(Oxoid, UK). Suspected colonies of MRSA and ESBL-E were identified by standard laboratory procedures.<br />

Results<br />

Screening samples were collected from 100 house pets, 42 cats (42%), 57 dogs (57%) and 1 guinea<br />

pig. Also nasal screening swabs for MRSA detection were collected from 6 veterinary workers (2 veterinary<br />

doctors and 4 assistants). We detected only 1 cat colonized with ESBL-E and no house pets<br />

colonized with MRSA. All screened employees of the veterinary clinic were free of MRSA.<br />

Conclusions<br />

Carriage of resistant bacteria in screened group of house pets was minimal. Since resistant bacteria<br />

can be easily transferred from owners to pets and vice versa periodic surveillance would seem<br />

reasonable.<br />

44

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