Large scale containment

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• Practices: BL2 + BL3 • Lab doors closed when work in progress • All activities in biosafety cabinet • No operation in open vessels conducted on benches • Use of plastic-backed paper toweling for easy cleaning • Dedicated protective clothes & change rooms, decontaminated prioe to laundering or disposal • Gowns including surgical masks • Respirator in rooms containing experimental animals • HEPA filtration of vacuum lines
BL3 • Facilities - equipment: BL2 + • Biological safety cabinets (cat. I, II or III) • Lab separated from areas of unrestricted traffic flow • Passage through a set of two doors • Double-door clothe change room with airlock • Shower optional • Wall, floor, ceiling impervious to water, acids, alkalis, organic solvents, moderate heat • Thru-wall pipework, cables etc… sealed • Foot-, elbow-operated or automatic hand wash station • Windows closed and sealed • Self-closing access doors • Decon autoclave preferably in the lab
Page 1 and 2: GMO and Biosafety Laboratory Produc
Page 3 and 4: NIH Guidelines “… any rDNA expe
Page 5 and 6: Laboratory Handling of Agents • G
Page 7 and 8: Containment Measures • Biological
Page 9 and 10: Physical Containment
Page 11 and 12: • Facilities - equipment BL1 •
Page 13: BL2 • Facilities - equipment: BL1
Page 17 and 18: BL4 • BL4 units (4 total in the w
Page 19 and 20: Large Scale • History (EU) • La
Page 21 and 22: BL1-Large Scale • = BL1 +: • Re
Page 23 and 24: BL2-Large Scale • = BL1-Large Sca
Page 25 and 26: • = BL2-Large Scale +: BL3-Large
Page 27 and 28: BL3-Large Scale (contd.) • Contro
Page 29 and 30: What Category is our Bp Strain?
Page 31 and 32: B. Pertussis (Cm R ) Cm R ptx (S2
Page 33 and 34: Our rec. B. pertussis strain? • E

Large scale containment

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