Large scale containment

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Large Scale: Principles • Primary containment • Closed systems: fermentor, bioreactor, Westfalia separator…, CIP-SIP • Open system in an enclosure: centrifuge, filtration system in biosafety cabinet… • Secondary containment • Controlled area design: airlocks, change rooms, negative pressure in controlled area, self closing doors, dedicated decontamination autoclave, exhaust air handling…
BL1-Large Scale • = BL1 +: • Reporting of spills and accidents resulting in overt exposure of organisms reported to Lab Director; medical evaluation, surveillance and treatment of staff as appropriate • Anything >10 L must be in a closed system; smaller cultures (seeding etc…) can be handled outside a closed system provided all BL1 physical containment requirements are met • Culture fluid (including samples…) shall not be removed from a closed system unless inactivated by a validated inactivation procedure • Sample collection from-, addition of materials to a closed system conducted to minimize aerosol & contamination
Page 1 and 2: GMO and Biosafety Laboratory Produc
Page 3 and 4: NIH Guidelines “… any rDNA expe
Page 5 and 6: Laboratory Handling of Agents • G
Page 7 and 8: Containment Measures • Biological
Page 9 and 10: Physical Containment
Page 11 and 12: • Facilities - equipment BL1 •
Page 13 and 14: BL2 • Facilities - equipment: BL1
Page 15 and 16: BL3 • Facilities - equipment: BL2
Page 17 and 18: BL4 • BL4 units (4 total in the w
Page 19: Large Scale • History (EU) • La
Page 23 and 24: BL2-Large Scale • = BL1-Large Sca
Page 25 and 26: • = BL2-Large Scale +: BL3-Large
Page 27 and 28: BL3-Large Scale (contd.) • Contro
Page 29 and 30: What Category is our Bp Strain?
Page 31 and 32: B. Pertussis (Cm R ) Cm R ptx (S2
Page 33 and 34: Our rec. B. pertussis strain? • E

Large scale containment

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