Large scale containment

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Wild type B. pertussis ptx (S1…S5) Cm R pSS4245::Gen R ptx promoter I-SceI gene I-SceI site Cointegrate (Str R, Cm R ) Cm R Str R Str R active in B. pertussis, NOT in E. coli Conjugative transfer, Str selection, ptx repression ptx induction DSB Wild type (~50%) Resolution Cm R (~50%) Cm R ptx (S2…S5)
B. Pertussis (Cm R ) Cm R ptx (S2…S5) S1* pSS4245::S1* ptx promoter I-SceI gene I-SceI site Cointegrate (Str R , Cm R ) Str R active in B. pertussis, NOT in E. coli Conjugative transfer, Str selection, ptx repression Str R ptx induction DSB Cm R Cm R (~50%) Resolution S1*, Cm S (~50%) ptx (S1*…S5)
Page 1 and 2: GMO and Biosafety Laboratory Produc
Page 3 and 4: NIH Guidelines “… any rDNA expe
Page 5 and 6: Laboratory Handling of Agents • G
Page 7 and 8: Containment Measures • Biological
Page 9 and 10: Physical Containment
Page 11 and 12: • Facilities - equipment BL1 •
Page 13 and 14: BL2 • Facilities - equipment: BL1
Page 15 and 16: BL3 • Facilities - equipment: BL2
Page 17 and 18: BL4 • BL4 units (4 total in the w
Page 19 and 20: Large Scale • History (EU) • La
Page 21 and 22: BL1-Large Scale • = BL1 +: • Re
Page 23 and 24: BL2-Large Scale • = BL1-Large Sca
Page 25 and 26: • = BL2-Large Scale +: BL3-Large
Page 27 and 28: BL3-Large Scale (contd.) • Contro
Page 29: What Category is our Bp Strain?
Page 33 and 34: Our rec. B. pertussis strain? • E

Large scale containment

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