Large scale containment

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• NIH scope Our rec. B. pertussis strain? • Irreversible loss of active pertussis toxin, an important virulence factor • However, B. pertussis produces several other toxic and non-toxic virulence factors which are still present: dermonecrotic toxin, tracheal cytotoxin, adenylate cyclase/hemolysin, FHA, pertactin… Bp 9K-129G remains a RG II pathogen Production in BL2-<strong>Large</strong> Scale
Our rec. B. pertussis strain? • EU scope • Probably not subject to EU directive on the contained use of GMO: “Self-cloning consisting in the removal of nucleic acid sequences from a cell of an organism which may or may not be followed by reinsertion of all or part of that nucleic acid (or a synthetic equivalent) with or without prior enzymic or mechanical steps into cells of the same species…” Bp 9K-129G has to be treated as wt B. pertussis Production in BL2-<strong>Large</strong> Scale
Page 1 and 2: GMO and Biosafety Laboratory Produc
Page 3 and 4: NIH Guidelines “… any rDNA expe
Page 5 and 6: Laboratory Handling of Agents • G
Page 7 and 8: Containment Measures • Biological
Page 9 and 10: Physical Containment
Page 11 and 12: • Facilities - equipment BL1 •
Page 13 and 14: BL2 • Facilities - equipment: BL1
Page 15 and 16: BL3 • Facilities - equipment: BL2
Page 17 and 18: BL4 • BL4 units (4 total in the w
Page 19 and 20: Large Scale • History (EU) • La
Page 21 and 22: BL1-Large Scale • = BL1 +: • Re
Page 23 and 24: BL2-Large Scale • = BL1-Large Sca
Page 25 and 26: • = BL2-Large Scale +: BL3-Large
Page 27 and 28: BL3-Large Scale (contd.) • Contro
Page 29 and 30: What Category is our Bp Strain?
Page 31: B. Pertussis (Cm R ) Cm R ptx (S2

Large scale containment

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