Large scale containment
Large scale containment
Large scale containment
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
Wild type B. pertussis<br />
ptx (S1…S5)<br />
Cm R<br />
pSS4245::Gen R<br />
ptx promoter<br />
I-SceI gene<br />
I-SceI site<br />
Cointegrate (Str R, Cm R )<br />
Cm R<br />
Str R<br />
Str R active in B. pertussis, NOT in E. coli<br />
Conjugative transfer, Str selection,<br />
ptx repression<br />
ptx induction<br />
DSB<br />
Wild type (~50%)<br />
Resolution<br />
Cm R (~50%)<br />
Cm R<br />
ptx (S2…S5)