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Presentation 22<br />

TECHNIQUES FOR CURRENT CONTROL IN TANK; LESSONS LEARNED FROM<br />

REARING LARVAE OF RED SPOTTED GROUPER Epinephelus akaara<br />

Takashi Iwasaki *1 , Masatsugu Takano 2 , Kazuhisa Teruya 3<br />

1 Stock Enhancement Technology Development Center, National Research Institute of<br />

Aquaculture, FRA, Saiki, Oita 879-2602, Japan<br />

2 Research Management Department, Headquarters, FRA, Minato-Mirai, Nishi-ku, Yokohama<br />

220-6115, Japan<br />

3 Stock Enhancement Technology Section, Ishigaki Tropical Station, Seikai National Fisheries<br />

Research Institute, FRA, Fukai-Ohta, Ishigaki, Okinawa 907-0451, Japan<br />

* Email: tak0829 at affrc.go.jp<br />

Mass mortality of the early larval-stage (from hatch to about 10 days after hatching) has been one<br />

of the big constraints during the process of mass fry production in groupers (Epinephelus bruneus,<br />

E. septemfasciatus), bluefin tuna (Thunnus orientalis) or amberjacks (Seriola dumerili, S.<br />

quinqueradiata). A phenomenon associated with the mass mortality is observed that early stage<br />

larvae sink to <strong>and</strong> pile onto tank bottom, <strong>and</strong> the phenomenon, so called “sinking death<br />

syndrome”, has been supposed to be one of the causes of the mass mortality. Many marine fish<br />

appear to show ontogenetic change of larval body density, <strong>and</strong> it is speculated that body density<br />

<strong>and</strong> swimming activity of larvae <strong>and</strong> current condition of rearing tank mutually influence on the<br />

incidence of sinking death. It is indicated that proper current condition is important to prevent the<br />

sinking death syndrome, <strong>and</strong> aeration, submersible pump <strong>and</strong>/or wave-making device have been<br />

practically examined to make the current proper.<br />

In the present study, rearing experiments were conducted in 60 m 3 tanks to investigate the<br />

effects of current control by aeration volumes for preventing the sinking death syndrome of red<br />

spotted grouper larvae. The aeration was done through four porous air hoses (1.5 m in length, 25<br />

mm in diameter) that were set on the tank bottom along the lower edge of each wall. Larvae were<br />

reared different aeration rates (0.25-1.50 L/min/tube), <strong>and</strong> the distribution, feeding amount,<br />

survival <strong>and</strong> growth of larvae were determined. Larvae in the tank with aeration rate of 0.25<br />

L/min/tube showed clearly the sinking performance to tank bottom. Larvae with aeration rate of<br />

1.50 L/min/tube were not remarkably distributed in the lower layer, showing the best survival<br />

without any differences in growth <strong>and</strong> feeding amounts from larvae in the other treatments. Thus,<br />

the present results clearly show that physical environments such as water current inside tanks are<br />

crucial factors to the improvement of survival <strong>and</strong> consequently the establishment of stable/mass<br />

fry production technologies in groupers.<br />

44

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