Program and Abstracts(PDF)

Poster presentation 13
IMMUNE RESPONSE AGAINST EDWARDSIELLA TARDA IN GINBUNA
CRUCIAN CARP (CARASSIUS AURATUS LANGDORFII)
Masatoshi Yamasaki 1* , Kyosuke Araki 2 , Mitsuru Ototake 3 , and Atsushi Yamamoto 2 ,
1
Graduate study of Fisheries, Kagoshima University, Shimoarata, Kagoshima,
Kagoshima, 890-0056, Japan
2
Faculty of Fisheries, Kagoshima University, Shimoarata, Kagoshima, Kagoshima,
890-0056, Japan
3
National Research Institute of Aquaculture, Fisheries Research Agency, Minamiise,
Mie 516-0193, Japan
* E-mail: goodspeed04013 at yahoo.co.jp
Edwardsiella tarda is an intracellular pathogen with a broad host range and causes
Edwardsiellosis in fish. Although there is no direct evidence, previous studies have
suggested that cell-mediated immunity (CMI) has a major role in the protection from
intracellular bacterial infection in fish, as in mammals. In order to verify the induction
of CMI, immune response was evaluated in fish after infection with E. tarda.
Ginbuna crucian carp (Caraccius auratus langsdrfii) were infected with E. tarda at
3.4*10 4 CFU/fish by intraperitoneal injection (control; PBS). Kidney leukocytes (KLs)
were isolated after experimental infection and examined expression level of
interferon-gamma (IFN-gamma) gene, perforin gene, CD8alpha gene and TCR-beta
gene for monitoring CMI.
Results of the gene expression analysis, the expression level of all isotypes of
IFN-gamma in KLs showed increase at 2 days post-experimental infection. In addition
the expression level of IFN-gamma1-1 had decreased at 4days was up-regulated from
8days to 16days as compared to control. Expression of perforin-1 and perforin-3 were
suppressed by infection with E. tarda. In contrast expression of perforin-2, CD8alpha
and TCR-beta were up-regulated from 8 days to 16 days as compared to control. These
results suggest that CMI was induced in the protection from infection with E. tarda.
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