Program and Abstracts(PDF)
Program and Abstracts(PDF)
Program and Abstracts(PDF)
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Poster presentation 12<br />
MOLECULAR IDENTIFICATION OF ANISAKIS SIMPLEX FROM FISHES<br />
CAUGHT ALONG THE COAST OF KAGOSHIMA AND HUMAN GASTRIC<br />
ANISAKIASIS<br />
Hiroshi Kita 1* , Kyosuke Araki 2 , Erlinda R. Cruz-Lacierda 2 , <strong>and</strong> Atsushi Yamamoto 2<br />
1 Graduate school of Fisheries, Kagoshima University, Shimoarata, Kagoshima, Kagoshima,<br />
890-0056, Japan<br />
2 Faculty of Fisheries, Kagoshima University, Shimoarata, Kagoshima, Kagoshima, 890-0056,<br />
Japan<br />
* Email: loupsgarouseye at yahoo.co.jp<br />
Anisakis simplex is known as a parasitic nematode of human gastric anisakiasis. There have<br />
been 2000~3000 infection reports every year in Japan. Morphological identification using<br />
the third stage larvae (L3 larvae) can only determine type groupings ; Anisakis Type Ⅰ larvae<br />
or Anisakis Type Ⅱ larvae. Anisakis Type Ⅰ has longer ventriculus <strong>and</strong> has mucro while<br />
Anisakis Type Ⅱ has short ventriculus <strong>and</strong> has no mucro. On the other h<strong>and</strong>, recent<br />
molecular studies of Anisakis, which launched from a series of studies using allozyme <strong>and</strong><br />
mitochondrial DNA (mt DNA) cox2 gene analyses, revealed that the Anisakis species<br />
consisted of 8 valid species, 5 species [A. ziphidarum, A. typica <strong>and</strong> three sibling species of A.<br />
simplex complex, namely A. simplex (sence stricto, s.s.), A. pegreffii, <strong>and</strong> A. simplex C] <strong>and</strong><br />
3species [A. paggiae, A. brevispiculate <strong>and</strong> A. physeteris] of which are in the Anisakis Type<br />
Ⅰ <strong>and</strong> Anisakis Type Ⅱ groupings, respectively.<br />
Recent study showed A. simplex identified from fish caught along the coast of Japan consisted<br />
of A. simplex (s.s.) <strong>and</strong> A. pegreff, the former is dominant in fishes caught along the Pacific<br />
coast of Japan, the latter is dominant in fishes caught in the Japan Sea. The aim of this study is<br />
examined the species composition of A. simplex collected from fishes caught along the coast<br />
of Kagoshima <strong>and</strong> from patients with gastric anisakiasis. L3 larvae were collected from the<br />
visceral of anchovy (Engraulis japonicus), chub mackerel (Scomber japonicus), bluefin tuna<br />
(Thunnus thynnus) caught along the coast of Kagoshima in 2010. Anisakis larvae agent of<br />
anisakiasis were collected from patients in Kagoshima, Miyazaki <strong>and</strong> Kumamoto from 2009<br />
to 2010.All Anisakis Type Ⅰ larvae were examined by PCR-RFLP of the ITS region<br />
(ITS1-5.8-ITS2) of ribosomal DNA.<br />
As a result, 38 individuals of Anisakis Type Ⅰ larvae were collected from those fishes, 10<br />
were identified as A. simplex (s.s.), 27 identified as A. pegreffii, <strong>and</strong> 1 identified as A. typica.<br />
12 individuals of collected from anisakiasis patients <strong>and</strong> all were identified as A. simplex<br />
(s.s.).<br />
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