Program and Abstracts(PDF)

Poster presentation 4
CHARACTERIAZATION OF CLASS B SCAVENGER RECEPTOR FROM KURUMA
SHRIMP MARSUPENAEUS JAPONICUS
Raja Sudhakaran 1* , Tohru Mekata 2 , Shogo Okugawa 3 , Mari Inada 3 , Maki Yoshimine 3 , Junichi
Nishi 3 , Tomoya Kono 4 , Masahiro Sakai 1 and Toshiaki Itami 1
1
Faculty of Agriculture, University of Miyazaki, 1-1, Gakuen Kibanadai-nishi, 889-2192
Miyazaki, Japan
2 Aquatic Animal Health Division, National Research Institute of Aquaculture, Fisheries
Research Agency, Saiki, 879-2602 Oita, Japan
3
Graduate School of Agriculture, University of Miyazaki, 1-1, Gakuen Kibanadai-nishi, 889-
2192 Miyazaki, Japan
4
Interdisciplinary Research Organization, University of Miyazaki, 1-1, Gakuen Kibanadai-nishi,
889-2192 Miyazaki, Japan
* Email: sudha at cc.miyazaki-u.ac.jp
The scavenger receptor, Croquemort is a member of the CD36 superfamily comprising
transmembrane proteins involved in the recognition of polyanionic ligands. It has been proved
by various researchers that member of CD36 superfamily are involved in immunity and
developmental processes.
In the present study, we report a cDNA encoding the kuruma shrimp, Marsupenaeus japonicus
Croquemort scavenger receptor (MjSCRBQ) obtained from the cDNA library of lymphoid organ
and RACE amplification. The full length cDNA of 2098 bp contains an open reading frame of
1596 nucleotides that translates into a 532-amino acid putative peptide putative peptide, with a 5’
untranslated region of 323 bp and 3’ UTR of 153 bp.
The MjSCRBQ is constitutively expressed in gills, heart, hemolymph, hepatopancreas, intestine,
lymphoid organ, muscle, nerve, stomach and in the brain with high value. Expression analysis in
lymphoid organs of WSSV post infection revealed the high levels of MjSCRBQ in 72 and 120
hrs durations. The MjSCRBQ contains putative functional domains including transmembrane
domains and CD36 domain.
Multiple alignments of MjSCRBQ amino acid sequences showed significant identity with
DmSCRBQ (31%), SsSCRBQ (29%), HsSCRBQ (28%) and RnSCRBQ (30%). In the
phylogenetic analysis, MjSCRBQ is identified in the invertebrate scavenger receptor region.
This is the first report in crustacean sector on identification and characterization of Croquemort
scavenging receptor, MjSCRBQ which may lead to better understanding of the molecular events
unexplored in shrimp system.
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