Program and Abstracts(PDF)
Program and Abstracts(PDF)
Program and Abstracts(PDF)
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Poster presentation 7<br />
ASTAKINE, AN ANCIENT CYTOKINE, IN KURUMA SHRIMP: RNA<br />
EXPRESSION AND SEQUENCE ANALYSIS OF MJASTAKINE GENE<br />
Mari Inada 1* , Shogo Okugawa 1 , Junichi Nishi 1 , Maki Yoshimine 1 , Raja Sudhakaran 2 , Tomoya<br />
Kono 3 , Thoru Mekata 4 , Terutoyo Yoshida 2 , Masahiro Sakai 2 <strong>and</strong> Toshiaki Itami 2<br />
1 Graduate School of Agriculture, University of Miyazaki, 1-1, Gakuen Kibanadai-nishi,<br />
Miyazaki, 889-2192 Japan<br />
2 Faculty of Agriculture, University of Miyazaki, 1-1, Gakuen Kibanadai-nishi, Miyazaki,<br />
889-2192 Japan<br />
3 Interdisciplinary Research Organization, University of Miyazaki, 1-1 Gakuenkibanadai-nishi,<br />
Miyazaki, 889-2192 Japan<br />
4 National Research Institute of Aquaculture, Fisheries Research Agency, Tsuiura, Kamiura,<br />
Saiki, Oita Pref. 879-2602, Japan<br />
* Email:agg902u at student.miyazaki-u.ac.jp<br />
Cytokines have been known to regulate hematopoiesis <strong>and</strong> immune responses in vertebrates.<br />
However, information about cytokines or cytokine-like factors concerning the hematopoiesis<br />
was very few in invertebrates, although the importance of the hemocytes has been well known<br />
in crustacean immune responses. Astakine was found to induce the shrimp hematopoietic<br />
stem cell differentiation as the first cytokine-like factor in crustacean. We present here the<br />
entire cDNA sequence (1,589 bp) of the kuruma shrimp Marsupenaeus japonicus astakine<br />
(Mjastakine).<br />
The open reading frame of Mjastakine encoded a protein of 124 amino acids with an<br />
estimated mass of 13.3 kDa. Amino acid sequence homology of Mjastakine were 79.8% <strong>and</strong><br />
52.4% to these of the tiger shrimp Penaeus monodon <strong>and</strong> freshwater crayfish Pacifastacus<br />
leniusculus. In the prokineticin domain of crustaceans, 11 cysteines were highly conserved.<br />
Mjastakine mRNA was highly expressed in the brain <strong>and</strong> hemocytes in healthy shrimp. On<br />
the other h<strong>and</strong>, Mjastakine mRNA was low level expression in hepatopancreas <strong>and</strong> ovary. In<br />
the hemocytes, Mjastakine expression reached its peak one day <strong>and</strong> decreased to its normal<br />
level 5 days after the virus injection when white spot syndrome virus (WSSV) was injected<br />
into the kuruma shrimp. This study was supported, in-part, by the University of Miyazaki's<br />
<strong>Program</strong> for the Support of Women in the Sciences <strong>and</strong> the Research <strong>and</strong> Development<br />
<strong>Program</strong> for New Bio-industry Initiatives <strong>and</strong> Japan Society for the Promotion of Science.<br />
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