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Matsuyama M., T. Shiraishi, J.K. Sundaray, M.A. Rahman, K. Ohta, and A. Yamaguchi. 2005. Steroidogenesis in ovarian follicles of chub mackerel, Scomber japonicus. Zool. Sci. 22: 101-110. Shiraishi T., K. Ohta, A. Yamaguchi, M. Yoda, H. Chuda, and M. Matsuyama. 2005. Reproductive parameters of the chub mackerel Scomber japonicus estimated from human chorionic gonadotropin induced final oocyte maturation and ovulation in captivity. Fish. Sci. 71: 531-542. Shiraishi T., K. Okamoto, M. Yoneda, T. Sakai, S. Ohshimo, S. Onoe, A. Yamaguchi, and M. Matsuyama. 2008. Age validation, growth and annual reproductive cycle of chub Scomber japonicus off the waters of northern Kyushu and in the East China Sea. Fish. Sci. 74: 947-954. Shiraishi T., S.D. Ketkar, Y. Katoh, M. Nyuji, A. Yamaguchi, and M. Matsuyama. 2008. Spawning frequency of the Tsushima Current subpopulation of chub mackerel Scomber japonicus off Kyushu, Japan. Fish. Sci. 75: 649-655. 67
Poster presentation 3 CHARACTERIZATION OF A NEWLY IDENTIFIED ANTI-LIPOPOLYSAHHARIDE FACTOR (ALF2) GENE IN KURUMA SHRIMP, MARSUPENAEUS JAPONICUS Shogo Okugawa 1* , Mari Inada 1 , Junichi Nishi 1 , Maki Yoshimine 1 , Raja Sudhakaran 2 , Terutoyo Yoshida 2 , Tohru Mekata 3 , Yoshihiro Suzuki 4 , Tomoya Kono 5 , Masahiro Sakai 2 and Toshiaki Itami 2 1 Graduate School of Agriculture, University of Miyazaki, Japan 2 Faculty of Agriculture, University of Miyazaki, 1-1, Gakuenkibanadai-nishi, Japan 3 National Research Institute of Aquaculture, Fisheries Research Agency, Japan 4 Faculty of Engineering, University of Miyazaki, Japan 5 Interdisciplinary Research Organization, University of Miyazaki, Japan * Email: agg905u at student.miyazaki-u.ac.jp Innate immunity is the important defense mechanism of invertebrates against harmful agents. Antimicrobial peptides (AMPs) play significant role in innate immunity of shrimp in the forms of anti-lipopolysaccharide factors (ALFs), crustins, penaeidins and lysozymes. AMPs have broad spectrum of antimicrobial activities such as killing or neutralizing bacteria, fungi, parasites and viruses. So far, ALF genes have been found in different species of shrimp includes Penaeus monodon (PmALF), Fenneropenaeus chinensis, Litopenaeus setiferus, L. vannamei, L. stylirostris and Marsupenaeus japonicus (MjALF1). More than one isoform have been identified in P. monodon, and representation of MjALF1 was up-regulated upon stimulation with LPS in vitro. In this study, we have identified a new ALF isoform from kuruma shrimp M. japonicus (MjALF2). The full-length MjALF2 gene consists of 558 bp with a 363 bp open reading frame, encoding 121 amino acids, which contains a putative signal peptide of 22 amino acids. The deduced amino acid sequence of MjALF2 showed 83.3% identity with sequences of PmALF2. Phylogenetic analysis revealed MjALF2 had been placed in the group closer to PmALF1 and PmALF2. Expression analysis revealed that MjALF2 had been constitutively expressed in brain, gill, heart, hemocytes, hepatopancreas, intestine, lymphoid organ, muscle, nerve, stomach. This study was supported by Research and Development Program for New Bio-industry Initiatives and Japan Society for the Promotion of Science. 68
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UJNR Aquaculture Panel 39th Scienti
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Fuminari Itoh (National Research In
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Walton W. Dickhoff (NOAA, Northwest
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Satellite Symposium in Shibushi 29
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Presentation 2 AQUACULTURE RESEARCH
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Presentation 4 AQUALCULTURE POLICY
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Presentation 5 AQUACULTURE IN NORTH
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Presentation 6 OVERCOMING TECHNICAL
Page 17 and 18: NOAA. 2008. Offshore Aquaculture in
Page 19 and 20: Presentation 8 SPAWNING AND LARVAL
Page 21 and 22: increase in tissue cortisol levels.
Page 23 and 24: 1)Hamasaki, K., Tsuruoka, K. Teruya
Page 25 and 26: Presentation 11 OFFSHORE MUSSEL FAR
Page 27 and 28: Presentation 12 STRUCTURAL PROBLEMS
Page 29 and 30: Presentation 14 FISH NUTRITION AND
Page 31 and 32: contaminate levels in aquacultured
Page 33 and 34: ANNOTATED BIBLIOGRAPHY OF KEY WORKS
Page 35 and 36: Presentation 17 BIOTECHNOLOGY FOR C
Page 37 and 38: Presentation 19 BIOINDICATOR AND BI
Page 39 and 40: environmental deterioration in and
Page 41 and 42: Weirich, C.R., P.S. Wills, R.M. Bap
Page 43 and 44: Presentation 21 LANDBASED POLY-ECO-
Page 45 and 46: Presentation 22 TECHNIQUES FOR CURR
Page 47 and 48: Presentation 23 POND-TO-PLATE ANALY
Page 49 and 50: TABLE 1. In-Pond Raceway System - p
Page 51 and 52: Presentation 24 BENEFICIAL MEAT QUA
Page 53 and 54: ANNOTATED BIBLIOGRAPHY OF KEY WORKS
Page 55 and 56: Satellite symposium 1 ARTIFITIAL CO
Page 57 and 58: Satellite symposium 2 SPAWNING AND
Page 59 and 60: Yoseda K., K. Yamamoto, K. Asami, M
Page 61 and 62: Satellite symposium 4 SABLEFISH HAT
Page 63 and 64: Program of Poster Presentations The
Page 65 and 66: Poster presentation 1 SOURCE AND SU
Page 67: Annotated Bibliography of Key Works
Page 71 and 72: Poster presentation 5 IDENTIFICATIO
Page 73 and 74: Poster presentation 7 ASTAKINE, AN
Page 75 and 76: Poster presentation 9 COMMUNITY STR
Page 77 and 78: Poster presentation 11 ASSESMENT OF
Page 79 and 80: Poster presentation 13 IMMUNE RESPO
Page 81 and 82: Poster presentation 15 EFFECTS OF P
Page 83 and 84: Poster presentation 17 EFFECTS OF D
Page 85 and 86: Poster presentation 19 SEDIMENT CHA
Page 87 and 88: IMP was synergistic rather than add
Page 89: Poster presentation 21 HOW DO WE RE
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