The Students' Guide in Quantitative Analysis - Free Ebooks Library

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QUANTITATIVE ANAJLVSfS.
Stopper which is connected by tubing with the measuring
tube. The urine is now mixed with the hypobromite, and
the disengaged nitrogen is driven into the measuring tube.
The tube is now levelled to relieve hydrostatic pressure,
and the vobime of nitrogen read off. Since 55 c. c. equal
o. 1 5
grm. of urea, a single division corresponds to ^—
30
= 0.005 grm. urea. .
(0.15 grm. urea gives 55 c. c. nitrogen at 60° Fah- and
30' bar.)
7. Determination of Actual Ammonia. Take 20 c. c.
filtered urine and treat by Schlosing's method.
The NH3 is expelled by milk of lime, and absorbed by
standard acid, in the cold under a bell jar. For details see
Frcs. § 99, 3 b. (Human urine contains 0.078 to 0.143 per
cent.)
8, Determination of Albumen. Measure urine
passed in 24 hours. Drop 50 c. c, one c. c. at a time,
into I ounce boiling distilled water in a porcelain dish.
If the urine was alkaline add a drop of acetic acid, avoid
excess. Allow the coagulated albumen to .settle, filter
through a weighed filter, and waslv well Dry at 100° C,
and weigh.
_
9- Determination of Sugar. Dilute urine 5 or 10
times, and apply Fehling's solution as in grape sugar. See
Analysis No. 35, Rmv Sugar.
10. Determination of Phosphoric Acid. To 50 c. c.
hltered urme add 5 c. c. sodic acetate and titrate with
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Analysis
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11. Determination of Uric Acid. _ To -00 c c
uruie add 10 c. c. HCI =;t-iiirl ^q k^ ,1*
»-. ijv^i, stand 4S hours m a cool place,
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