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Phytopathogenic Dothideomycetes - CBS - KNAW

Phytopathogenic Dothideomycetes - CBS - KNAW

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Verkley et al.<br />

Fig. 10. Septoria astragali, <strong>CBS</strong> 109116. A–C. Colonies (15 C, nUV). A. On OA. B. On CHA. C. On MEA. D. Conidia and conidiogenous cells on OA (<strong>CBS</strong> 109116); E–G. Conidia<br />

in planta (<strong>CBS</strong> H-21258). H. Conidiogenous cells on OA (<strong>CBS</strong> 123878). I. Conidia on OA (<strong>CBS</strong> 123878). Scale bars = 10 µm.<br />

walls covered with short mycelial outgrowth, with a single opening<br />

releasing a stout cirrhus of pale whitish to rosy-buff conidial slime;<br />

reverse mostly olivaceous-black due to the conidiomata; after<br />

incubation of 5–7 wk, more of the immersed mycelium darkens<br />

to olivaceous-black, with traces of a red pigment especially near<br />

the margin, and the aerial mycelium becomes more dominant,<br />

white or grey. Colonies on CMA 2–3 mm diam in 10 d (27–28 mm<br />

in 7 wk), as on OA, but the reddish pigment at the margin more<br />

conspicuous in old cultures. Colonies on MEA 1.5–3 mm diam in 10<br />

d ((8–)14–17 mm in 7 wk), with an even to irregular, glabrous, buff<br />

margin; colonies first restricted, while later faster growing hyphal<br />

strands colonize the medium underneath the surface of the agar,<br />

pustulate to hemispherical, the surface first ochreous or amber,<br />

later olivaceous-grey or black covered by fairly dense, short,<br />

white aerial mycelium; some superficial or immersed pycnidial<br />

conidiomata formed, releasing cirrhi of pale buff conidial slime;<br />

reverse dark umber to brown-vinaceous. Colonies on CHA 1.5–3<br />

mm diam in 10 d (15–17 mm in 7 wk), with an irregular margin<br />

which is hardly visible from above; colonies restricted, irregularly<br />

pustulate to hemispherical, the surface dark brick to dark slate<br />

244

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