Xenogen IVIS-200 user manual

More documents

Recommendations

Info

10. Auto ROIsFigure 10.4 An overlapping ROI (ROI 1) with a 20% contour created using theAuto 1 option.In addition to being able to create auto ROIs defined as Contour, usersmay create auto Circle or Square ROIs. A Circle ROI is an ellipse thatbest fits the shape of the contour and is of equal area. A Square ROI isa rectangle created using the major and minor axes of the ellipsecorresponding to a Circle ROI.Auto ROIs allow the user to define an ROI in a consistent, nonsubjectivemanner. For example, an ROI with a 10% contour around themaximum peak edge value is completely defined and easilyreproducible. Consistent use of auto ROIs with a defined edge valuewill result in better quantification of most experimental data than othermethods.10.2 Subject ROIsSubject ROIs are rectangular-shaped boxes that enclose a particular,often single, subject animal. Auto creation of Subject ROIs attempts tolocate individual animals in a photographic image and enclose theanimal in a Subject ROI. Default Auto Subject ROI settings areoptimized to locate light-colored animals on a dark background.However, the settings may be varied to give good results for subjectanimals of different types. Settings values are discussed in more detailin Chapter 4, page 21.Another use of Subject ROIs is that of creating a group box in which allROIs contained or placed in the box are assigned the propertiesassociated with the Subject ROI, e.g., if a background ROI is associatedwith a subject ROI, all measurement ROIs in the subject ROI will beassociated with this background. See Section 9.1, page 80.Xenogen is working to extend the usefulness of Subject ROIs,specifically, giving Living Image software the ability to locate, withina number of stored images, the image data associated with oneparticular animal. These potential Subject ROIs would be assigned aparticular Subject ID, or identifier, that could be used to find, e.g.,Mouse 123456, in a set of multiple images. See ROI Properties panel,page 35. Presently, Living Image software allows users to create subjectanimal IDs, but does not yet provide the ability to locate them across allimages.90
Living Image ® Software ManualFigure 10.5 Auto creation of three Subject ROIs, one for each of animals in thephotographic image.91
Page 4:
© 2002-2006 Xenogen Corporation. A
Page 8 and 9:
1. WelcomeCCD cameraImaging chamber
Page 10 and 11:
1. WelcomeImaging chamberCCDcameraO
Page 12 and 13:
1. Welcomeis used to indicate pull-
Page 14 and 15:
2. Quick Start Guide to Acquiring I
Page 16 and 17:
3. Control Panel for IVIS ® Imagin
Page 18 and 19:
Page 20 and 21:
Page 22 and 23:
Page 24 and 25:
Page 26 and 27:
Page 28:
4. Image Display and Analysis Windo
Page 31 and 32:
Living Image ® Software Manualallo
Page 33 and 34:
Living Image ® Software ManualFigu
Page 35 and 36:
Living Image ® Software Manualwher
Page 37 and 38:
Living Image ® Software Manualback
Page 39 and 40:
Living Image ® Software ManualFigu
Page 41 and 42:
Living Image ® Software ManualSubs
Page 43 and 44:
Living Image ® Software ManualBack
Page 45 and 46: 5Sequence WindowLiving Image ® Sof
Page 47 and 48: Living Image ® Software Manualcont
Page 49 and 50: Living Image ® Software Manual6 Ma
Page 51 and 52: Living Image ® Software ManualEach
Page 53 and 54: Living Image ® Software Manualset
Page 55 and 56: Living Image ® Software ManualFor
Page 57 and 58: Living Image ® Software Manualduri
Page 59 and 60: Living Image ® Software ManualMeas
Page 61 and 62: Living Image ® Software ManualFigu
Page 63 and 64: Living Image ® Software Manualchec
Page 65 and 66: Living Image ® Software ManualDisp
Page 67 and 68: Living Image ® Software ManualSmoo
Page 71 and 72: Living Image ® Software ManualClic
Page 73 and 74: Living Image ® Software ManualManu
Page 75 and 76: Living Image ® Software Manual7Ima
Page 77 and 78: Living Image ® Software Manualinfo
Page 79 and 80: 8Sensitivity and BinningLiving Imag
Page 81 and 82: Living Image ® Software Manualhard
Page 83 and 84: Living Image ® Software ManualFina
Page 85 and 86: 9Living Image ® Software ManualMea
Page 89 and 90: Living Image ® Software Manualhow
Page 93 and 94: Living Image ® Software Manual10 A
Page 95: Living Image ® Software Manualthe
Page 99 and 100: Living Image ® Software Manual11Ba
Page 101 and 102: Living Image ® Software Manualimag
Page 103 and 104: Living Image ® Software ManualBeca
Page 105 and 106: Living Image ® Software Manual12Da
Page 107 and 108: Living Image ® Software Manual12.3
Page 109 and 110: Living Image ® Software Manual13Da
Page 111 and 112: Living Image ® Software Manualsear
Page 113 and 114: 14Fluorescent ImagingLiving Image
Page 117 and 118: Living Image ® Software ManualBeca
Page 121 and 122: Living Image ® Software ManualTiss
Page 123 and 124: Living Image ® Software ManualUnde
Page 127 and 128: Living Image ® Software Manualmost
Page 129 and 130: Living Image ® Software ManualCorr
Page 131 and 132: Living Image ® Software Manualauto
Page 133 and 134: Living Image ® Software Manual15 S
Page 139 and 140: Living Image ® Software Manualtime
Page 143 and 144: Living Image ® Software ManualThe
Page 145 and 146: Living Image ® Software Manual16 S
Page 147 and 148:
Living Image ® Software Manuala. T
Page 149 and 150:
Living Image ® Software Manualc.Th
Page 151 and 152:
Living Image ® Software ManualInde
Page 153 and 154:
Living Image ® Software Manual100
show all

Xenogen IVIS-200 user manual

Create successful ePaper yourself

Delete template?

Save as template?