Protein-Protein Interactions, Networks and Pathways - Chagall

Reliability of PPI• PPI interactions are identified by variousexperimental techniques with varying degreesof accuracies.• High-throughput interactions are inherently‘noisy’. Large variations betweenexperiments, sparse overlap with knowninteractions.• Many abundant ‘sticky’ proteins (e.gRibosome, chaperones).• Experimental techniques are continuouslyimproving. New datasets are more reliable.• References - vonMering et al., 2002; Sprinzak et al., 2003;Bader et al. 2002; Siato et al., 2003; Jansen et al.; 2003, Denget al.; 2003; Bader et al., 2004; Gandhi et al.,2006 and more…