Protein-Protein Interactions, Networks and Pathways - Chagall
Protein-Protein Interactions, Networks and Pathways - Chagall
Protein-Protein Interactions, Networks and Pathways - Chagall
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Yeast-two-hybrid• Widely used for detectingPPI.• Can detect weakinteractions (Kd~10 -7 )• Often detect spuriousinteractions.• Excludes membrane <strong>and</strong>secreted proteins thatcannot be detected by anuclear-based system.• Some TF are self-activating.• Review - Fields S., FEBS J.,2005, 272,5391-7
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