engineering design day 2012 - College of Engineering @ The ...
engineering design day 2012 - College of Engineering @ The ...
engineering design day 2012 - College of Engineering @ The ...
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DEVELopmENT oF VERY quICk poLYmERASE ChAIN REACTIoN (pCR) DEVICE<br />
Agricultural and Biosystems <strong>Engineering</strong><br />
CLASS<br />
ABE 498A/B<br />
SpoNSoR<br />
UA Biosensors Lab, Jeong-Yeol<br />
Yoon’s Research Group<br />
SpoNSoR mENToR/ADVISoR<br />
Dr. Jeong-Yeol Yoon<br />
pRoJECT mENToR<br />
Dr. Jeong-Yeol Yoon<br />
Dr. David You<br />
Dustin Harshman<br />
TEAm mEmBERS<br />
Ramon Muñoz (ABE)<br />
Franklin Garcia (ABE)<br />
Franklin Ventura (ABE)<br />
ABE = Agricultural and Biosystems<br />
<strong>Engineering</strong><br />
TEAm ABE-03: pRoJECT SummARY<br />
Using <strong>design</strong>s and methods developed<br />
in Dr. Yoon’s Lab the team has created<br />
a very quick Polymerase Chain<br />
Reaction (PCR) device. PCR is a wellestablished<br />
method for the detection<br />
and amplification <strong>of</strong> DNA and RNA<br />
used in medical research and clinical<br />
medicine to detect infectious diseases<br />
and gene mutations. PCR uses three<br />
steps—denaturing, annealing, and<br />
extension—to amplify specific DNA<br />
sequences <strong>of</strong> interest. Current commercial PCR devices require two hours or more<br />
to complete the process. <strong>The</strong> method developed in this <strong>design</strong> reduces the time to<br />
less than 10 minutes and allows for real-time detection.<br />
Specific Design Goals:<br />
• Optimize/Manufacture heating circuit & PID control<br />
• Miniaturize circuit board<br />
• Develop coating for device<br />
• Develop real-time detection method<br />
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