PDF File - The Indian Society for Parasitology

Two new species of Trypanosoma59After thorough washing, the films were air dried, Kinetoplast: Well developed structure and takes deepmounted in DPX, sealed and observed and stain upon fixation. Shape may be conical or spherical.photographed at 1000 x magnification under LEICA Division of the kinetoplast not observed.DMLB photoautomat. Camera lucida drawings weremade for statistical measurements of the parasites.Flagellum and undulating membrane: Flagellumoriginates from kinetoplast, runs anteriorly borderingNuclear index (NI), flagellar index (FI) and the undulating membrane and finally extends beyondkinetoplast index (KI) were calculated as follows: body as a free flagellum. Flagellum well-developed inNI = PN/NA; FI = CL/FL; KI = PN/KNlarger forms than smaller forms. Undulatingmembrane conspicuous and takes a light stain. Itwhere PN = posterior end to nucleus ; NA = anterior extends throughout length of cell body. In smallerend to nucleus ; CL = body length ; FL = flagellar forms, it has about 1-2 folds, whereas in larger forms itlength and KN = kinetoplast to nucleus.has 1-3 folds. Width of undulating membrane greaterin larger forms.RESULTSIndicesTrypanosoma saulii n.sp. (Fig. 1 A-D; Table 1)FI = 1.92Host - Channa punctatus Bloch KI = 1.72Locality - Bans Mandi, Bareilly, India NI = 1.62Percentage ofinfection/intensity - 10.6%/1-12.4DISCUSSIONTrypanosomes/100 RBCsThe genus Channa (=Ophiocephalus) has, from time(n = 115)to time, been reported as a suitable host for protozoanThe live parasite in hanging drop preparation showed parasites (Haldar and Mukherjee, 1979). Variousan active wriggling movement amongst the blood trypanosome species have been put on record and acorpuscles. Out of the infected fish, 6.4% werecomparison indicates that T. ophicephali, T. punctatimoderately infected (parasitaemia 5-12.4and T. elongatus are monomorphic forms and,Trypanosomes/100 RBCs), the remaining had scantinfection (parasitaemia 1-5 Trypanosomes/100ARBCs). In stained smears, two forms of the parasiteBcould be differentiated : minuta and magna.C1. MorphologyDShape: Trypanomastigote elongated, anterior andposterior ends tapering, the latter may assume a beaklikeappearance. Configuration of parasite usually ofletter 'C', sometimes elongated 'S' and in rare cases,almost a straight line. Posterior end extremity in both(small and large) cases, more or less rounded.Cytoplasm: Granular cytoplasm stains lightly. In thesmaller forms, the granules more concentrated in theanterior region of the body commencing from justanterior tip of the nucleus to approximately half thedistance from their commencement to the origin of theflagellum. In large forms, granules occur along bothsides of nucleus.Nucleus: Deep red stained nucleus oblong or beanshapedin middle of cell body, occupying almost entirecell breadth.EIGFig. 1. A-D T. saulii n. sp. (A, B: minuta froms; C, D: magnaforms), E-K T. heteropneusti n. sp. (E, F: smaller forms; G, H:larger forms; I-K: diving forms)J10 µmKFH