Regeneration Strategies for Streptavidin Biosensors on ... - ForteBio

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<strong>Regeneration</strong> <strong>Strategies</strong> <strong>for</strong> <strong>Streptavidin</strong> <strong>Biosensors</strong> on the Octet Systemsfrom binding cycle 1 to 11 (Figure 6A–D). Conditions HCl, glycinepH 1, glycine pH 2 and phosphoric acid all show good reproducibilityof the hIgG binding across all eleven cycles (Figure 6E–H). Inparticular, the 500 mM phosphoric acid (sensor H1) reagent showsvery good regeneration of the protein A surface with a high level ofreproducibility of hIgG binding throughout the assay.From this single experiment a regeneration condition of 3X10second cycles of 500 mM phosphoric acid has been identifiedas an excellent reagent to regenerate this receptor on <strong>Streptavidin</strong>biosensors.6
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