Chromatography - Bartelt

ChromatographyChromatography columns/HPLCLLG CHROMATOGRAPHY CATALOGUEAn optimised phase for every separationsApplication Similar phases** Separation principle ∙ Retention mechanismin general compoundswith ionizablefunctionalgroups such asbasic pharmaceuticalsand pesticideslike C 18 Gravity,however generallyshorter retentiontimes for nonpolarcompoundsNUCLEOSIL ® C 18 HDWaters Xterra ® RP 18 / MS C 18 ;Phenomenex Luna ® C18 (2),Synergi und Max RP; Zorbax ®Extend C18; Inertsil ® ODS III;Purospher ® RP-18, Star RP-18NUCLEOSIL ® C 8 HDWaters Xterra ® RP 8 / MS C 8 ;Phenomenex Luna ® C8;Zorbax ® Eclipse; XDB-C8only hydrophobicinteractions(van der Waalsinteractions)Si(CH3) 3H 3CCH 3NOhigh steric selectivity,thus suitedfor separation ofpositional andstructural isomers,planar / non-planarmoleculesbasic pharmaceuticalingredients,very polar compounds,organicacidscompounds witharomatic and multiplebond systemsrobust C 18 phasefor routineanalysesrobust C 8 phase forroutine analysesNUCLEOSIL ® C 18 ABInertsil ® ODS-P;YMC ® Pro C18RSPhenomenex Aqua ® ;YMC ® AQ;Waters Atlantis ® dC18no similar phasesNUCLEOSIL ® C 18Spherisorb ® ODS II; Hypersil ®ODS; Waters Symmetry ® C18;Inertsil ® ODS II; Kromasil ®C18; LiChrospher ® RP 18NUCLEOSIL ® C 8 ec / C 8Spherisorb ® C8; Hypersil ®MOS; Waters Symmetry ® C8;Kromasil ® C8; LiChrospher ®RP 8steric interactionsand hydrophobicinteractionshydrophobicinteractions andpolar interactions(H bonds)OHH 3CCH 3π-π interactionsand hydrophobicinteractions NO 2only hydrophobicinteractions(van der Waalsinteractions)some residual silanolinteractionsSi(CH 3) 3SiOHH 3CNCH 3NOOColumns for HPLCpolar organiccompounds (basicdrugs, moleculescontaining π electronsystemsNUCLEOSIL ® CN / CN-RPπ-π interactions,polar interactions(H bonds),hydrophobicinteractionsC NC NHOOsugars, sugar alcoholsand other hydroxycompounds,DNA bases, polarcompounds ingeneralNUCLEOSIL ®NH 2 / NH 2 -RPpolar / ionicinteractions,hydrophobicinteractions+NH 3OOHpolar organic compoundsin generalunmodified NUCLEOSIL ®polar / ionicinteractionsSiOHO 2N** phases which provide a similar selectivity based on chemical and physical properties58E & OE.