Columns for HPLC - Western Analytical

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Columns for HPLCColumns for HPLCAnalytical columns packed with NUCLEOSIL ®Octadecyl phases -(CH 2 ) 17 – CH 3Analysis of nitroaromatic compounds(explosives)Column 250 x 3 mm NUCLEOSIL ® 120-3 C 18 ; eluents: A) methanol,B) water, gradient 35 – 55% A in 12 min, then 40 min isocratic,finally 100% A, flow rate 0.35 ml/min; temperature 30 °C,detection DAD, 230 nm; injection volume 40 µlPeaks:1) 2,6-Diamino-4-nitrotoluene2) 2,4-Diamino-4-nitrotoluene3) Hexogene (Hexahydro-1,3,5-trinitro-1,3,5-triazine)4) 1,3,5-Trinitrobenzene5) 2-Methyl-3-nitroaniline6) 2-Methyl-5-nitroaniline7) 4-Methyl-3-nitroaniline200100mAU123456878) 1,3-Dinitrobenzene9) 2,4,6-Trinitrotoluene10)4-Amino-2,6-dinitrotoluene11)2-Amino-4,6-dinitrotoluene12)2,6-Dinitrotoluene13)2,4-Dinitrotoluene14)2-Nitrotoluene15)4-Nitrotoluene16)3-Nitrotoluene910 111312114810C 18 phases are suited for reversed phase chromatographyand ion-pairing chromatography for separation of nonpolar tomoderately polar compounds such asfatty acidsglyceridespolycyclic aromaticsesters (phthalates)fat-soluble vitaminssteroidsprostaglandinsPTH amino acids etc.Separation of triazines3 4 5Column: 250 x 4 mm NUCLEOSIL ® 50-5 C 18 ecEluent: methanol – acetonitrile – water (40:20:40, v/v/v)Flow rate: 1 ml/min, 30 °C, 220 barDetection: UV, 220 nmPeaks (injection volume 5 µl, 10 µg/ml each):1. Simazine 12. Atrazine3. Prometon 24. Ametryne5. Propazine6. Prometryne7. Terbutryne6 711157014 15 16020 40 mincourtesy of Dr. Steinbach, Inst. f. Org. Chemistry, Univ. Marburg010 20 minOrdering information · Analytical columns with NUCLEOSIL ® C 18Length → 30 mm 1) 70 mm 100 mm 125 mm 150 mm 250 mm Guard columnsNUCLEOSIL ® 50-5 C 18 ecParticle size 5 µm, pore size 50 Å; octadecyl phase, endcapped, 14.5% C; eluent in column acetonitrile / waterChromCart ® columns2 mm ID 721825.20 721826.20 721828.20 721829.303 mm ID 721825.30 721826.30 721828.30 721829.304 mm ID 721825.40 721826.40 721828.40 721829.404.6 mm ID 721825.46 721826.46 721827.46 721828.46 721829.40EC columns 3)4 mm ID 720098.40 721829.404.6 mm ID 720098.46 721829.4030 mm ChromCart ® columns and CC guard column cartridges (8 mm) in packs of 3, all other columns in packs of 1.1)30 mm ChromCart ® columns require the CC column holder 30 mm (Cat. No. 721823).2)On request, Microbore columns are also available in lengths of 40, 60, 200 and 300 mm and with 0.15, 0.3, 0.4, 0.5, 0.75 and 1.5 mm ID.Guard columns for Microbore columns on request.3)As guard columns for EC columns use ChromCart ® guard column cartridges with guard column adaptor EC (Cat. No. 721359).6.7.05Custom-packed columns with other phases and other lengths are available on requestMNwww.mn-net.com39
Columns for HPLCColumns for HPLCAnalytical columns packed with NUCLEOSIL ®Ordering information · Analytical columns with NUCLEOSIL ® C 18Length → 30 mm 1) 70 mm 100 mm 125 mm 150 mm 250 mm Guard columnsNUCLEOSIL ® 100-3 C 18Particle size 3 µm, pore size 100 Å; octadecyl phase, endcapped, 15% C; eluent in column acetonitrile / waterChromCart ® columns2 mm ID 721883.20 721865.20 721866.303 mm ID 721883.30 721865.30 721866.304 mm ID 721883.40 721865.40 721866.404.6 mm ID 721883.46 721806.46 721865.46 721866.40Microbore columns 2)1 mm ID 717029.10 717020.10 717011.10 717003.10EC columns 3)2 mm ID 720150.20 720133.20 721866.303 mm ID 720150.30 720133.30 721866.304 mm ID 720150.40 720133.40 721866.404.6 mm ID 720150.46 720949.46 720133.46 721866.40NUCLEOSIL ® 100-5 C 18Particle size 5 µm, pore size 100 Å; octadecyl phase, endcapped, 15% C; eluent in column acetonitrile / waterChromCart ® columns2 mm ID 721074.20 721781.20 721622.20 721662.20 721602.303 mm ID 721074.30 721781.30 721622.30 721662.30 721602.304 mm ID 721074.40 721781.40 721622.40 721662.40 721602.404.6 mm ID 721074.46 721781.46 721622.46 721642.46 721662.46 721602.40Microbore columns 2)1 mm ID 717028.10 717019.10 717010.10 717002.10EC columns 3)2 mm ID 720002.20 720014.20 721602.303 mm ID 720002.30 720014.30 721602.304 mm ID 720002.40 720014.40 721602.404.6 mm ID 720002.46 720120.46 720014.46 721602.40NUCLEOSIL ® 100-7 C 18Particle size 7 µm, pore size 100 Å; octadecyl phase, endcapped, 15% C; eluent in column acetonitrile / waterChromCart ® columns3 mm ID 721878.30 721609.304 mm ID 721878.40 721609.404.6 mm ID 721609.46EC columns 3)4 mm ID 720018.404.6 mm ID 720018.4630 mm ChromCart ® columns and CC guard column cartridges (8 mm) in packs of 3, all other columns in packs of 1.1)30 mm ChromCart ® columns require the CC column holder 30 mm (Cat. No. 721823).2)On request, Microbore columns are also available in lengths of 40, 60, 200 and 300 mm and with 0.15, 0.3, 0.4, 0.5, 0.75 and 1.5 mm ID.Guard columns for Microbore columns on request.3)As guard columns for EC columns use ChromCart ® guard column cartridges with guard column adaptor EC (Cat. No. 721359).6.7.0540Each column is individually tested and supplied with test chromatogram and test conditionswww.mn-net.comMN
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Columns for HPLC - Western Analytical

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