Columns for HPLC - Western Analytical

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Columns for HPLCColumns for HPLCAnalytical columns packed with NUCLEOSIL ®Ordering information · Analytical columns with NUCLEOSIL ® C 8Length → 30 mm 1) 70 mm 100 mm 125 mm 150 mm 250 mm Guard columnsNUCLEOSIL ® 120-3 C 8Particle size 3 µm, pore size 120 Å; octyl phase, not endcapped, 6.5% C; eluent in column acetonitrile / waterChromCart ® columns2 mm ID 721720.20 721786.20 721782.20 721785.303 mm ID 721720.30 721786.30 721782.30 721785.304 mm ID 721720.40 721786.40 721782.40 721785.404.6 mm ID 721720.46 721786.46 721722.46 721782.46 721785.40EC columns 3)2 mm ID 720071.20 720703.20 721785.303 mm ID 720071.30 720703.30 721785.304 mm ID 720071.40 720703.40 721785.404.6 mm ID 720071.46 720214.46 720703.46 721785.40NUCLEOSIL ® 120-5 C 8Particle size 5 µm, pore size 120 Å; octyl phase, not endcapped, 6.5% C; eluent in column acetonitrile / waterChromCart ® columns2 mm ID 721868.20 721892.20 721801.20 721787.303 mm ID 721868.30 721892.30 721801.30 721787.304 mm ID 721868.40 721892.40 721801.40 721787.404.6 mm ID 721868.46 721892.46 721521.46 721801.46 721787.40EC columns 3)2 mm ID 720050.20 720052.20 721787.303 mm ID 720050.30 720052.30 721787.304 mm ID 720050.40 720052.40 721787.404.6 mm ID 720050.46 720735.46 720052.46 721787.40NUCLEOSIL ® 300-5 C 8Particle size 5 µm, pore size 300 Å; octyl phase, not endcapped, ~3% C; eluent in column acetonitrile / waterChromCart ® columns3 mm ID 721103.30 721098.30 721101.304 mm ID 721103.40 721098.40 721101.40EC columns 3)4 mm ID 720062.40 721101.404.6 mm ID 720062.46 721101.4030 mm ChromCart ® columns and CC guard column cartridges (8 mm) in packs of 3, all other columns in packs of 1.1)30 mm ChromCart ® columns require the CC column holder 30 mm (Cat. No. 721823).2)On request, Microbore columns are also available in lengths of 40, 60, 200 and 300 mm and with 0.15, 0.3, 0.4, 0.5, 0.75 and 1.5 mm ID.Guard columns for Microbore columns on request.3)As guard columns for EC columns use ChromCart ® guard column cartridges with guard column adaptor EC (Cat. No. 721359).www.mn-net.com6.7.05MNCustom-packed columns with other phases and other lengths are available on requestwww.mn-net.com45
Columns for HPLCColumns for HPLCAnalytical columns packed with NUCLEOSIL ®Phenyl phases -(CH 2 ) 3C 6 H 5 phases are suited for reversed phase chromatographyand ion pairing chromatography for separation of moderatelypolar compounds. The retention characteristics are similar tothe C 8 phase, but with different selectivity for polycyclic aromatichydrocarbons, polar aromatics, fatty acids etc.Separation of opium alkaloids and meconic acidN.R. Ayyangar, S. R. Bhide, J. Chromatogr. 436 (1988) 455-465Column: 250 x 4 mm NUCLEOSIL ® 100-7 C 6 H 5Eluent A: methanol – water (5:95) with 1% triethylammoniumphosphate stock buffer, pH 3.2Eluent B: methanol – water (80:30) with 1% triethylammoniumphosphate stock buffer, pH 3.95Gradient: linear from 100% A to 100% B in 20 min,then 12 min eluent BFlow rate: 1,5 ml/minDetection: UV, 280 nmPeaks:1. Morphine2. Meconic acid3. Codeine4. Thebaine5. Laudanosine6. Cryptopine7. Noscapine8. Narceine9. Papaverine% v CH 3 OH10090807060504030201001230 2 4 6 8 10 12 14 16 18 20 22 24 26min45 6 7891012006.7.05Ordering information · Analytical columns with NUCLEOSIL ® C 6 H 5Length → 125 mm 150 mm 250 mm Guard columnsNUCLEOSIL ® 100-5 C 6 H 5 ecParticle size 5 µm, pore size 100 Å; phenyl phase, endcapped, 8 % C; eluent in column acetonitrile / waterChromCart ® columns3 mm ID 721535.30 721533.30 721537.304 mm ID 721535.40 721533.40 721537.40NUCLEOSIL ® 100-5 C 6 H 5Particle size 5 µm, pore size 100 Å; phenyl phase, not endcapped, 8% C; eluent in column acetonitrile / waterChromCart ® columns3 mm ID 721860.30 721861.30 721862.304 mm ID 721860.40 721861.40 721862.404.6 mm ID 721860.46 721887.46 721861.46 721862.40EC columns 1)2 mm ID 720695.20 721862.303 mm ID 720695.30 720956.30 721862.304 mm ID 720956.40 721862.404.6 mm ID 720956.46 721862.40ChromCart ® guard column cartridges (8 mm) in packs of 3, all other columns in packs of 1.1)As guard columns for EC columns use ChromCart ® guard column cartridges with guard column adaptor EC (Cat. No. 721359).Each column is individually tested and supplied with test chromatogram and test conditions46www.mn-net.comMN
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