Columns for HPLC - Western Analytical

Columns for HPLCColumns for HPLCAnalytical columns packed with NUCLEOSIL ®Diol phasesSFC separation of steroidsF. Vérillon et al. LC · GC int. 7 (1994) 710 – 716Column: 250 x 2 mm NUCLEOSIL ® 100-7 OH (Diol)Eluent: CO 2 with 1 – 12% methanolFlow rate: 2.5 ml/minOutlet pressure: 14 – 35 MpaETemperature: 85 °CDetection: UV, 210 nmPeaks:KA) CholesterolB) ProgesteroneGC) AndrosteroneA FD) TestosteroneHI JB DE) EstroneF) EstradiolCG) CortisoneH) HydrocortisoneI) PrednisoneJ) PrednisoloneK) Estriol109840-(CH 2 ) 3 – O – CH 2 – CH – CH 2–OH–OHOH phases are suited for reversed phase chromatographyand normal phase chromatography; they are less polar thanunmodified silica and very easily wettable with water.Please note: the eluent in the columns is n-heptane. If youwant to use an eluent which is not miscible with n-heptane(e. g. water), it is necessary to rinse the column with THFfirst.0 2 4 min 6The hollow in the upper trace is caused by instantaneous detection ofthe pressure gradient and delayed detection of the methanol gradient.The lower trace shows a software-corrected chromatogram.Ordering information · Analytical columns with NUCLEOSIL ® OH (Diol)Length → 125 mm 250 mm Guard columnsNUCLEOSIL ® 100-5 OH (Diol)Particle size 5 µm, pore size 100 Å; diol phase, eluent in column n-heptaneChromCart ® columns3 mm ID 721480.30 721471.30 721478.304 mm ID 721480.40 721471.40 721478.40EC columns 1)4 mm ID 720143.40 721478.404.6 mm ID 720143.46 721478.40NUCLEOSIL ® 100-7 OH (Diol)Particle size 7 µm, pore size 100 Å; diol phase, eluent in column n-heptaneEC columns 1)4 mm ID 720070.404.6 mm ID 720070.46ChromCart ® guard columns cartridges (8 mm) in packs of 3, all other columns in packs of 1.1) As guard columns for EC columns use ChromCart ® guard column cartridges with guard column adaptor EC (Cat. No. 721359).6.7.05Custom-packed columns with other phases and other lengths are available on requestMNwww.mn-net.com53