Columns for HPLC - Western Analytical

Columns for HPLCColumns for HPLCAnalytical columns packed with NUCLEOSIL ®Dimethylamino phases -(CH 2 ) 3 – N(CH 3 ) 2The DMA phase is a weakly basic anion exchanger for theseparation of many anions; it can also be used in a similarway as the NH 2 phase.Please note: the eluent in the columns is n-heptane. If youwant to use an eluent which is not miscible with n-heptane(e. g. water), it is necessary to rinse the column with THFfirst.Separation of sennosides from rhubarbY. Ohshima, K. Takahashi, J. Chromatogr. 258 (1983) 292 – 296Column: NUCLEOSIL ® 100-5 N(CH 3 ) 2 , 250 x 8 mm IDEluent: THF – water – acetic acid (8:2:1)Flow rate: 3,5 ml/minDetection: UV, 280 nmSamples: methanolic extracts ofa) Rheum palmatum var. tanguticumb) Rheum coreanumc) Rheum palmatumd) Rheum coreanum x Rheum palmatum (Shinshu-rhubarb)(d)(c)(b)(a)min 10 0 min 10 0 min 10 0 min 10 06.7.05102590Ordering information · Analytical columns with NUCLEOSIL ® N(CH 3 ) 2Length → 250 mm Guard columnsNUCLEOSIL ® 100-5 N(CH 3 ) 2Particle size 5 ± 1.5 µm, pore size 100 Å; dimethylamino phase, eluent in column n-heptaneEC columns4 mm ID 720994.40 721610.404.6 mm ID 720994.46 721610.40ChromCart ® guard column cartridges (8 mm) in packs of 3, all other columns in packs of 1.As guard columns for EC columns use ChromCart ® guard column cartridges with guard column adaptor EC (Cat. No. 721359).56Each column is individually tested and supplied with test chromatogram and test conditionswww.mn-net.comMN