Iowa Section of AADR - The University of Iowa College of Dentistry

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R01 DEO14390. Supported by: University of Iowa, College of Dentistry, Iowa Dental Research Grant 4. HBD3 Inhibits Porphyromonas gingivalis rHagB Binding to Dendritic Cells J. Van Hemert 5 , E. Recker 5 , K. Walters 2 , A. Progulske-Fox 6 , K.A. Brogden 5 2 University of Iowa, Iowa City, IA; 5 University of Iowa College of Dentistry, Iowa City, IA; 6 University of Florida, Gainesville, FL Human β-defensin 3 (HBD3) is a small, well-characterized peptide with broad antimicrobial activities and diverse innate immune functions. Previously, we found that HBD3 binds to recombinant Porphyromonas gingivalis dendritic cells. Objectives: Our objective was to determine if HBD3 binding to rHagB alters the binding of rHagB to the surface of human myeloid dendritic cells. Methods: To test this, human myeloid dendritic cells and mouse JAWS II cells were incubated with 0.1 μM rHagB, 1.0 μM HBD3+0.1 μM rHagB (10:1 molar ratio), 1.0 μM HBD3, or 0.1 M PBS, pH 7.2. After 5 minutes, with monoclonal MoAb 1858 to rHagB, polyclonal rabbit to rHagB, and polyclonal 500-P241 rabbit antibody to microscopy. Results: For confocal microscopy, 6.3 x 104 dendritic cells in chamber slides were incubated with rHagB and had Conclusion: Overall, these results strongly suggest that HBD3 binding to rHagB alters the binding of rHagB to the response of rHagB in dendritic cells. This work was supported by NIH, NIDCR grant R01 DEO14390. Supported by: NIH, NIDCR grant R01 DEO14390. 11 12
5. Effectiveness of Group Home Caregivers’ Training Regarding Oral Care Delivery S.E. Swenson 1 , K. Weber-Gasparoni 1 , F. Qian 1 , R.L. Ettinger 1 1 University of Iowa Purpose: The purpose of this study was to investigate the effectiveness of training on the behaviors of group home caregivers who provided oral hygiene care for individuals with special health care needs (SHCN). Methods: A 24-item survey was distributed to 884 caregivers employed at six care facilities in Iowa. Bivariate analyses and logistic regression models were used to analyze the data (alpha=.05). Results: that caregivers who reported receiving training at their current facility (yes versus no) on how to provide oral hygiene care for individuals with SHCN were more likely to brush the teeth of uncooperative consumers more frequently (P=.003), and feel more comfortable providing oral hygiene care for those who verbally and physically resisted oral care (P=.0003). Multivariable logistic regression analyses indicated that individuals who reported receiving training were more likely to brush (P P=.03) the teeth of cooperative consumers, continue oral care delivery when consumers physically refused the care (P=.0041), provide direct care to a higher number of individuals with SHCN on a weekly basis (P=.0209), and disagree that lack of training was a barrier to providing oral hygiene care (P of consumers perceived as uncooperative. Conclusions: Caregivers who reported receiving training on how to provide oral hygiene care to individuals with SHCN displayed more positive behaviors when compared to those who did not receive training. Supported by: Delta Dental of Iowa Foundation 6. Sphingolipids and Fatty Acids Alter Porphyromonas gingivalis Protein/Lipid Compositions C.L. Fischer 2 , K. Walters2 , D.V. Dawson 2 , D.R. Drake 2 , K.A. Brogden 2 , P.W. Wertz2 2 University of Iowa, Iowa City, IA Long chain bases: sphingosine, phytosphingosine, and dihydrosphingosine, and fatty acids: lauric acid and sapienic acid, commonly found on oral mucosa and on skin have potent antimicrobial activity for a variety of Gram-positive and Gram-negative bacteria including Porphyromonas gingivalis. Treatment with these lipids also induces striking ultrastructural damage to the overall bacterial cell and formation of unique intracellular inclusions in some bacteria. However, little is known about the mechanisms of antimicrobial action as well as the nature and the origin of the bacterial inclusions. Objectives: of bacteria following treatment with lipids; 2) We determined if bacteria exhibited cell-association of test lipids following exposure. Methods: P. gingivalis was treated with long chain bases or fatty acids and pelleted by centrifugation. For one treatment, whole-cell protein extracts were reduced and separated by SDS polyacrylamide gel electrophoresis and stained. For another treatment, lipids were extracted and separated by thin layer chromatography. Controls bases and fatty acids. Results: Of the long chain bases and fatty acids examined, sapienic acid induced the greatest alterations in the Conclusions: Mechanisms for these alterations are not yet known and are being explored. Long chain bases and fatty acids are present in cell pellets suggesting they may be associating with the bacterial membrane or accumulating in the cell, perhaps in the forming inclusions. Supported by: NIH/NIDCR RO1 DEO18032 and R01 DEO14390. 13 12
Page 1 and 2: 1 Iowa Section of the American Asso
Page 3 and 4: Table of Contents Letter from Dean
Page 5 and 6: Dental Research participants and Io
Page 7 and 8: Program Iowa Section of the America
Page 9 and 10: Pre-Doctoral, Graduate, and Post-Do
Page 11 and 12: Abstracts 1. Incidence of Signs and
Page 13: phenotypes are currently being eval
Page 17 and 18: 8. Three-Dimensional Soft Tissue As
Page 19 and 20: 12. Streptococcus mutans Glucan-Bin
Page 21 and 22: 16. Assessment of Convenience of Gl
Page 23 and 24: 20. Growth Relationships between th
Page 25 and 26: 23. The Effect of Maxillary Restric
Page 27 and 28: adhesive. Supported by: NIDCR Train
Page 29 and 30: 28. Use of IV Sedation in Periodont
Page 31 and 32: 32. Caregivers’ Perceived Comfort
Page 33 and 34: 36. Effects of GR siRNA on Prolifer
Page 35 and 36: Iowa Section of AADR - Presidents 1
Page 37: We extend grateful acknowledgment t

Iowa Section of AADR - The University of Iowa College of Dentistry

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