Iowa Section of AADR - The University of Iowa College of Dentistry

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22. Enhancement of Rat MSC Capabilities by Intracelluar Release of Estradiol N. Benassi 1 , Y. Yu 1 , V. Joshi 1 , N. Wei 1 , A.K. Salem 1 , L. Hong 1 1 University of Iowa Stem cell-based bone tissue engineering has emerged as an increasingly promising alternative approach for the bone marrow-derived MSCs are considered a potentially attractive cell source for this approach, the natural population of MSC is very limited and their differentiation capabilities are easily reduced following in vitro cell expansion. Thus, it is essential to develop strategies that can be used to improve the potential utilization of MSCs for clinical application. Estrogen effectively improves MSC capabilities and has strong potential as a regulator of can serve as a tool for intracellular delivery of estradiol (E2) to effectively execute estrogen regulation of MSCs. Objectives: This study is to evaluate in vitro and in vivo bone regenerative capability of MSCs with intracellular release of E2. Methods: After rat bone marrow MSCs took up E2-loaded PLGA microparticles, their proliferation and in vitro In vivo bone regeneration of these MSCs was investigated using a rat calvarial bone defect model. Results: proliferation rate compared to the controls. mRNA expression of osteocalcin, an osteogenic differentiation marker, differentiation medium in vitro. Bone regeneration at calvarial defects treated by MSCs with uptake of E2-loaded PLGA particles are faster than controls. Conclusions: Intracellular release of E2 delivered by PLGA microparticles effectively enhanced proliferation and bone regenerative capabilities of MSCs. This approach can be potentially used to improve the capabilities of MSCs for MSC-based bone tissue engineering. Supported by: University of Iowa, College of Dentistry, Iowa Dental Research Grant 21 22
23. The Effect of Maxillary Restriction on Mandibular Fossa Morphology J. Kelly 1 , N.E. Holton 1 , S.D. Marshall 1 , R. Franciscus 1 , T.E. Southard 1 1 University of Iowa Objectives: In previous analyses, we found that experimentally-induced maxillary restriction in a pig model resulted in predictable changes in mandibular condylar morphology. In the present study we extend our analysis to assess the potential effects of maxillary restriction on the morphology of the mandibular fossa. Methods: Ten female domestic pig sibship cohorts, each consisting of three individuals, were allocated to one of months. The sham group underwent surgery, receiving only screw implantation, and the control group underwent no surgical procedure. Pigs were euthanized at six months of age. Mandibular fossa length and breadth were assessed using the Mann-Whitney U-test. Additionally, we assessed variation in mandibular fossa size and shape via geometric morphometric analysis of coordinate landmark data describing the fossa. To assess these data, we used Procrustes analysis, which distills a form, represented by coordinate landmark data, into size and shape information. Shape differences were quantitatively assessed using principal components (PC) of Procrustes scaled coordinate landmarks. Results: differences in shape. The results of our principal components indicated that the experimental group, relative to the control and sham groups, had a more superiorly positioned joint surface with lateral displacement relative to (from our previous analysis) describing differences in mandibular condylar morphology (r=0.49; p=0.012). Conclusions: This suggests that altering maxillary growth may have important consequences on masticatory function and joint biomechanics. Supported by: University of Iowa, College of Dentistry, Iowa Dental Research Grant 24. Effect of Benzalkonium Chloride Adhesive System on Resin-Dentin Bond Durability A. Murray 5 , M.A. Vargas 5 , S. Geraldeli 9 , F. Qian 8 , L. Chen 7 5 University of Iowa College of Dentistry, Iowa City, IA; 7 Research and Development, Bisco, Schaumburg, IL; 8 Department of Preventive and Community Dentistry, University of Iowa, Iowa City, IA,; 9 Restorative Dental Sciences, University of Florida, Gainesville, FL Objectives: The resin-dentin bond is degraded by endogenous dentinal proteinases activated by acidic compounds contained in dental adhesive systems. Several compounds have been suggested to neutralize these proteinases, including benzalkonium chloride (BAC). The objective is to evaluate the effect of incorporating various concentrations of BAC on the short and intermediate bond strength of a one-step self-etch adhesive. Methods: Forty-eight human molars stored in 0.5% chloramine solution were used within 3mo of extraction and randomly assigned to 4 groups (n = 12). Flat dentin surfaces were prepared with a water-cooled, low-speed diamond saw (Isomet1000, Buehler). A standardized smear layer was prepared using 600-grit wet sandpaper. Three experimental one-step self-etching adhesives (Bisco Inc.) were formulated with 0.1%, 0.2% and 0.5% BAC following manufacturer’s guidelines. A three increment build-up of approximately 5mm was made using resin composite Estelite Sigma Quick (Tokuyama). Each increment was light cured (690-730 mW/cm 2 ) for 20sec with sectioned to obtain six rectangular sticks from the central part of the bonded surface with a cross section area of approximately 0.8mm. At random, two sticks from each group were subjected to tension at a crosshead speed of 1.0mm/min using a Zwick testing machine at 1wk and two sticks at 10wks. Results: A two-way repeated measures ANOVA was used to evaluate differences among the groups by adhesive and 23 22
Page 1 and 2: 1 Iowa Section of the American Asso
Page 3 and 4: Table of Contents Letter from Dean
Page 5 and 6: Dental Research participants and Io
Page 7 and 8: Program Iowa Section of the America
Page 9 and 10: Pre-Doctoral, Graduate, and Post-Do
Page 11 and 12: Abstracts 1. Incidence of Signs and
Page 13 and 14: phenotypes are currently being eval
Page 15 and 16: 5. Effectiveness of Group Home Care
Page 17 and 18: 8. Three-Dimensional Soft Tissue As
Page 19 and 20: 12. Streptococcus mutans Glucan-Bin
Page 21 and 22: 16. Assessment of Convenience of Gl
Page 23: 20. Growth Relationships between th
Page 27 and 28: adhesive. Supported by: NIDCR Train
Page 29 and 30: 28. Use of IV Sedation in Periodont
Page 31 and 32: 32. Caregivers’ Perceived Comfort
Page 33 and 34: 36. Effects of GR siRNA on Prolifer
Page 35 and 36: Iowa Section of AADR - Presidents 1
Page 37: We extend grateful acknowledgment t

Iowa Section of AADR - The University of Iowa College of Dentistry

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