FINAL PROGRAM 6TH EDITION - EuroMediCom

food allergies and/or intolerance in the light of the reduced number of possible food allergens presented to the patient during the VLC-HP diet. Additionally, we looked into the evidence from genetic cohort studies of overweight and diabetic populations.Results: Cardiovascular risk factors (blood lipids, insulin resistance and hypertension) improve significantly more in HP diets,especially as compared to current pharmacological-only treatments and/or general dietary advice. A significant number of patientsexperiences resistance to weight loss from their genetic background, endocrine imbalances or intolerance of food components asassessed by IgG/IgE micro-array. Individual adjustment of the composition of the diet is mandatory according to these preliminaryfindings.Discussion: In our experience, high-protein (HP) diets are well tolerated and induce rapid and sustained loss of weight over andbeyond low caloric carbohydrate-based alternatives. Their added benefit centres primarily around their improved adherence. That isattributable to the prolonged HP-induced satiety and the lower incidence of gastro-intestinal intolerance. In the light of preliminary IgGdetermination, it is also very likely due to the low content and low variety of allergens.Conclusions: Maximally effective treatment of overweight and/or obesity should be tailor-made. It depends on the findings fromgenetic profiling, the comprehensive assessment of dietary habits, IgG-mediated food intolerances, endocrine screening, physicalactivity and psychological assessment. When planning a specific individualized treatment system in order to maximize outcome, thesemultiple etiological factors all need to be taken into account. In combination with behavioural guidance, VLC-HP diets are probably themost effective tool currently available. Their outcome can be further improved by adapting their detailed composition in accordancewith the results of extensive preliminary laboratory screening.SALIVARY HORMONE TESTING: DO’S AND DON’TSTESTS HORMONAUX SALIVAIRES : QUE FAIRE ET NE PAS FAIREWOLFGANG ZIEMANN(GERMANY)The HERS studies I and II as well as the WHI/NIH study have shown that HRT might result in elevated health risks for postmenopausalwomen. Therefore compounding pharmacies now are offering individualized HRT based on natural hormones and on the results ofsalivary hormone analysis. A recent publication has shown that the results of some commercial saliva labs are not reproducible.Therefore these results cannot be the basis of an individualized HRT.The main problem in salivary testing seem to be the pre-analytical procedures. Chewing and other pressure on the teeth will increasethe interference with plasma components and will result in elevated salivary hormone measurements. Visible blood contamination willdo the same and therefore must be avoided.The collection device must be free of adsorption effects. This especially is important for progesterone analysis.Therefore polypropylene is the best material for collection. Polyethylene should not be used at all. As meat and dairy products maycontain significant hormone concentrations such foods should not be consumed during the collection day.The main problem seems to be the significant biological CV of hormone secretion. The episodic secretion pattern does not allowreproducible results if saliva analysis is done just from one single sample. In order to reduce the biological variation of testing resultsany salivary measurement should be based on a mixture of 5 samples collected during a period of 2 to 3 hours.In order to catch the morning peak of Cortisol secretion the samples have to be collected during the first 2 hours after the usual wakeuptime.The maximum value usually will appear 1 hour after the usual awakening.In general salivary testing reflects the free hormone fraction and therefore the hormone activity. In contrast to this serum analysis onlycan give a result for the total hormone concentration which represents mainly (>95%) the inactive bound hormone fraction.Therefore salivary hormone testing is much superior to serum testing provided the correct pre-analytical procedures are carefullyfollowed.SESSION 15AAANTI AGING WORKSHOP 6 GENETIC TYPINGLE GÉNOTYPEANTI-AGING MEDICINE4.30 pm / 16h30MEN AND WOMEN: HOW TO WORK WITH THE MOST IMPORTANT GENETIC TYPING TESTS?HOMMES ET FEMMES : COMMENT TRAVAILLER AVEC LES MEILLEURS TESTS GÉNOTYPIQUES ?JOHANNES HUBER (AUSTRIA)ANDRES KANSKI (VENEZUELA)5-alpha reductase polymorphism is important for men and women in a different way. 5-alpha dehydrotestosteron protect the femalebreast, whereas it is a burden for the male prostate gland. Progesterone is converted in 2 metabolic high ways in the femaleorganism: In 3 pregnane and in 5 alpha-pregnene. The later one is associated with strong proliferation in the female breast andshould be avoided in the menopausal area.Aromatase gen display also different SNP, with higher enzyme activity, producing more estrogens from androgens. This is importantfor the female breast as also for pars medialis in the prostatic tissue.Catechol-O-Methyltransferase gen and methylen tetrahydrofolat-reductase gen have a faster and a slower working genetic variation,this important for cardiovascular system, for the mood and for the intestinal tract. Glucose metabolism and inflammation areconnected with aging process and display also a genetic variation in the competent genes, which becomes more and more importantfor preventive medicine.110