Bioinformatics for DNA Sequence Analysis.pdf - Index of

148 Creevey and McInerney2. Program UsageClann (37) is a command-line software package for investigatingphylogenetic information through supertree analyses and isfreely available under a GNU public license agreement. Version3.1 implements five different supertree methods, includingMRP, average consensus and the MSSA. In this version ofclann the MRP criterion requires the use of an external parsimonyprogram like PAUP* (38); future versions will remove thisrequirement.2.1. Installation Clann is available at http://bioinf.nuim.ie/software/clann. Onthe download page the choice of three different operating systemsare available (Mac OSX, Linux and Microsoft Windows). In MacOSX an installation script is included, which installs the readlineand ncurses libraries (if needed) before putting clann into thefolder /usr/bin/. Once in this location (and the user starts anew terminal window) clann will be visible to the operating systemfrom any directory. An administrative password will be needed tosuccessfully install clann.On a Linux operating system, the clann program should eitherbe located in the same directory as the input files, or somewhere onyour path (e.g. /bin/ or /usr/local/bin). If you do not knowwhich directories are on your path, ask your system administrator.On the Microsoft Windows double clicking on the icon associatedwith clann will run the program. Using this operatingsystem, the clann program must be located in the same directoryas the input files, an alias or shortcut to clann will not suffice.To run clann on the MacOSX or Linux operating systems, typethe command ‘‘./clann’’ or ‘‘clann’’ in a terminal window.2.2. File Formats Clann accepts source trees in two different formats: newick (alsocalled phylip format) and nexus format (see Note 1). Multiple treescan be contained in the same file in both formats. Newick-formattedtrees are the simplest to construct and can contain branchlengths, internal branch labels, tree weights and tree names (seeNote 2) (Fig. 7.4).The nexus format is a modular system for representing manytypes of systematic information, including sequences and trees(39). Branch lengths and internal branch labels are indicated inthe same manner to newick-formatted files. Different types ofsystematic information are contained within ‘‘blocks.’’ It is alsopossible to include a clann block, containing the commands to beexecuted on the data in the file. To load a file of trees into clann usethe command exe filename or include the name of the file to beexecuted along with the call for clann at the operating system