Scharlab - AMCO Instruments, SRL

www.scharlab.comHPLC Scharlau columnsKromaPhase Scharlau HPLC columnsFor over 30 years, Spanish scientists have been workingwith SCHARLAU HPLC columns. Pharmaceutical Labs.,Research Centres, Universities, Independent labs... haveall been using SCHARLAU columns due to their reliabilityand reproducibility.Each column is tested after manufacture for efficiency,capacity, selectivity and peak symmetry. The results ofthese tests are shown in the Test Chromatogram, which isincluded with each column.The wise choiceWe are especially proud of our latest development: TheKromaPhase column range.KromaPhase C18 is based on an ultra pure high performancespherical silica, providing high reproducibility and chemicalstability by using monofunctional silanes and full end-capping.KromaPhase C18 is stable from pH 1 to 10. Without changingexisting methods KromaSIL C18 can be replaced byKromaPhase C18. To guarantee batch reproducibility eachbatch undergoes specific tests.New TestColumn dimensions: 150 x 4,6 mmMobile phase: MeOH/20mM Phosphate Buffer pH 7, 65/35 (v/v)Flow rate: 1,4 mL/minDetection: UV @ 210 nmTemperature: 23°CSample: 1 Uracil, 2 Propranol, 3 Butyl Parabene, 4 Dipropyl phthalate,5 Naphthalene, 6 Acenaphthene, 7 Amitriptyline Without changing existing methodsKromaSIL C18 can be replacedby KromaPhase C18Engelhardt TestColumn dimensions: 150 x 4,6 mmMobile phase: MeOH/H2O 49/51 (w/w)Flow rate: 1,4 mL/minDetection: UV @ 254 nmTemperature: 40°CSample: 1 Uracil, 2 Aniline, 3 Phenol, 4 Impurity,5 p-Ethyl aniline, 6 Dimethyl aniline, 7 Toluene, 8 EthylbenzeneHPLC Scharlau columns– Kromasil C18– KromaPhase C18– Kromasil C18– KromaPhase C18Tanaka Test A: Shape Selectivity and HydrophobicityColumn dimensions: 150 x 4,6 mmMobile phase: MeOH/H2O 75/25 (w/w)Flow rate: 1,4 mL/minDetection: UV @ 254 nmTemperature: 40°CSample: 1 Uracil, 2 Butylbenzene, 3 o-Terphenyl4 Pentylbenzene, 5 Triphenylene– Kromasil C18– KromaPhase C18Tanaka Test B: Silanophilic ActivityColumn dimensions: 150 x 4,6 mmMobile phase: MeOH/TRIS-Buffer pH 7 30/70 (w/w)Flow rate: 1,4 mL/minDetection: UV @ 254 nmTemperature: 40°CSample: 1 Uracil, 2 Benylamine, 3 Phenol– Kromasil C18– KromaPhase C18Scharlab The Lab Sourcing Group359