PAS x40 - RCPA

Assessment of the glomerulus in electron micrographs 1:• mesangial matrix and basement membrane materialhave a similar electron density and texture• identify mesangial cell nuclei and cytoplasm– there should be only one mesangial cell nucleus in anymesangial area• look for mesangial matrix abnormalities– is there an increase?– are there immune complexes deposits, which are moreelectron dense (darker) than matrix and basementmembrane material?– are there aggregates of abnormal proteins such asamyloid, fibrillary and immunotactoid deposits with atexture or substructure?