PAS x40 - RCPA

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Sections & stains for light microscopy:Native renal biopsy– cut 24 serial sections• H&E stains x5• OVG x1(elastin)• <strong>PAS</strong> x2• Masson x1• AgMT x2• store the spare sectionsTransplant renal biopsy– cut 12 serial sections• H&E stains x4• OVG x1(elastin)• <strong>PAS</strong> x2• Masson x1• AgMT x2• store the spare sections
Immunohistochemical stains:Native• IgA• IgG• IgM• fibrin(ogen)• C3• C1qTransplant (Tx)• IgA• IgG• IgM• fibrin(ogen)• C3• C1q• C4d• BKV
Page 3: Laboratory handling of renal Bx:•
Page 7 and 8: Our Medical Laboratory Scientist re
Page 9 and 10: Tissue for light microscopy:• des
Page 11: A paraffin embedded renal biopsy co
Page 15 and 16: Examination of H&E stains:• on lo
Page 17 and 18: Medium power examination of H&E sta
Page 19 and 20: High power examination of H&E stain
Page 21 and 22: H&E x2This is an H&E stain of a ren
Page 23 and 24: H&E x10This is a higher power view.
Page 25 and 26: H&E x10This is renal medulla. Most
Page 27 and 28: marksglomerulimarks arteriolesmarks
Page 29 and 30: H&E x40marksglomeruli/Bowman’s ca
Page 31 and 32: H&E x40marks arteriolesoutlines par
Page 33 and 34: tubular polevascular pole
Page 35 and 36: peritubular capllariesarteriolessma
Page 37 and 38: H&E x2H&E x10This is a biopsy from
Page 39 and 40: atrophic tubules in anarea of inter
Page 41 and 42: segmental sclerosisfocal mild incre
Page 43 and 44: the mesangium is expandedby amorpho
Page 45 and 46: PAS x10
Page 47 and 48: Bowman’s capsulemesangiumtubular
Page 49 and 50: PAS x40Glomerulus sectioned through
Page 51 and 52: segmental sclerosisfocal hyalinosis
Page 53 and 54: OVG x40 - interlobulararteryOVG x40
Page 55 and 56: Masson x40 -normal glomerulusMasson
Page 57 and 58: Masson x40Masson x40variations in s
Page 59 and 60: Masson x40: glomerulus with mesangi
Page 61 and 62: AgMT x40 - small arteryAgMT x40 - g
Page 63 and 64:
AgMT x100Can you identify mesangial
Page 65 and 66:
This biopsy is from someonewith the
Page 67 and 68:
AgMT x100 -variations in staining f
Page 69 and 70:
mesangial cell nucleismallsubepithe
Page 71 and 72:
AgMT x100:there is fuzzy materialor
Page 73 and 74:
Congo red stain showing theapple gr
Page 75 and 76:
Examination of immunohistochemical
Page 77 and 78:
IF IgG x40Normal kidney -immunofluo
Page 79 and 80:
IF fibrin x40Normal kidney -immunof
Page 81 and 82:
IF C1q x40Normal kidney -immunofluo
Page 83 and 84:
IP IgA x40Normal kidney - immunoper
Page 85 and 86:
IP IgG x40Normal kidney - immunoper
Page 87 and 88:
IP IgM x40Normal kidney - immunoper
Page 89 and 90:
IP C3 x10
Page 91 and 92:
IP C1q x40IP C1q x10Normal kidney -
Page 93 and 94:
IP kappa x40IP lambda x40Immunopero
Page 95 and 96:
IP C4d x40Immunoperoxidase for C4d
Page 97 and 98:
IP SV40 x40 biopsy BImmunoperoxidas
Page 99 and 100:
Electron microscopy:• a piece of
Page 101 and 102:
Toluidine blue x40Toluidine blue x2
Page 103 and 104:
Assessment of the glomerulus in ele
Page 105 and 106:
This is a low power electron microg
Page 107 and 108:
This glomerulus is normocellular, w
Page 109 and 110:
mesangial matrixepithelial cellfoot
Page 111 and 112:
The Banff Classification of Renal A
Page 113 and 114:
Specimen adequacy(a necessary prere
Page 115 and 116:
Quantitative criteria for mononucle
Page 117 and 118:
Quantitative criteria for arteriola
Page 119 and 120:
Quantitative criteria for peritubul
Page 121 and 122:
Quantitative criteria for interstit
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Quantitative criteria for fibrous i
Page 125 and 126:
C4d scoring :% biopsy area(cortex a
Page 127 and 128:
2. Antibody-mediated changes:may co
Page 129 and 130:
4. T-cell-mediated rejection (TCMR)
Page 131 and 132:
6. Other:• changes not considered
Page 133 and 134:
References:• Banff 07 Classificat
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PAS x40 - RCPA

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