- Page 3:
Laboratory handling of renal Bx:•
- Page 7 and 8:
Our Medical Laboratory Scientist re
- Page 9 and 10:
Tissue for light microscopy:• des
- Page 11 and 12:
A paraffin embedded renal biopsy co
- Page 13 and 14:
Immunohistochemical stains:Native
- Page 15 and 16:
Examination of H&E stains:• on lo
- Page 17 and 18:
Medium power examination of H&E sta
- Page 19 and 20:
High power examination of H&E stain
- Page 21 and 22:
H&E x2This is an H&E stain of a ren
- Page 23 and 24:
H&E x10This is a higher power view.
- Page 25 and 26:
H&E x10This is renal medulla. Most
- Page 27 and 28:
marksglomerulimarks arteriolesmarks
- Page 29 and 30:
H&E x40marksglomeruli/Bowman’s ca
- Page 31 and 32:
H&E x40marks arteriolesoutlines par
- Page 33 and 34:
tubular polevascular pole
- Page 35 and 36:
peritubular capllariesarteriolessma
- Page 37 and 38:
H&E x2H&E x10This is a biopsy from
- Page 39 and 40:
atrophic tubules in anarea of inter
- Page 41 and 42:
segmental sclerosisfocal mild incre
- Page 43 and 44:
the mesangium is expandedby amorpho
- Page 45 and 46:
PAS x10
- Page 47 and 48:
Bowman’s capsulemesangiumtubular
- Page 49 and 50:
PAS x40Glomerulus sectioned through
- Page 51 and 52:
segmental sclerosisfocal hyalinosis
- Page 53 and 54:
OVG x40 - interlobulararteryOVG x40
- Page 55 and 56:
Masson x40 -normal glomerulusMasson
- Page 57 and 58:
Masson x40Masson x40variations in s
- Page 59 and 60:
Masson x40: glomerulus with mesangi
- Page 61 and 62:
AgMT x40 - small arteryAgMT x40 - g
- Page 63 and 64:
AgMT x100Can you identify mesangial
- Page 65 and 66:
This biopsy is from someonewith the
- Page 67 and 68: AgMT x100 -variations in staining f
- Page 69 and 70: mesangial cell nucleismallsubepithe
- Page 71 and 72: AgMT x100:there is fuzzy materialor
- Page 73 and 74: Congo red stain showing theapple gr
- Page 75 and 76: Examination of immunohistochemical
- Page 77 and 78: IF IgG x40Normal kidney -immunofluo
- Page 79 and 80: IF fibrin x40Normal kidney -immunof
- Page 81 and 82: IF C1q x40Normal kidney -immunofluo
- Page 83 and 84: IP IgA x40Normal kidney - immunoper
- Page 85 and 86: IP IgG x40Normal kidney - immunoper
- Page 87 and 88: IP IgM x40Normal kidney - immunoper
- Page 89 and 90: IP C3 x10
- Page 91 and 92: IP C1q x40IP C1q x10Normal kidney -
- Page 93 and 94: IP kappa x40IP lambda x40Immunopero
- Page 95 and 96: IP C4d x40Immunoperoxidase for C4d
- Page 97 and 98: IP SV40 x40 biopsy BImmunoperoxidas
- Page 99 and 100: Electron microscopy:• a piece of
- Page 101 and 102: Toluidine blue x40Toluidine blue x2
- Page 103 and 104: Assessment of the glomerulus in ele
- Page 105 and 106: This is a low power electron microg
- Page 107 and 108: This glomerulus is normocellular, w
- Page 109 and 110: mesangial matrixepithelial cellfoot
- Page 111 and 112: The Banff Classification of Renal A
- Page 113 and 114: Specimen adequacy(a necessary prere
- Page 115 and 116: Quantitative criteria for mononucle
- Page 117: Quantitative criteria for arteriola
- Page 121 and 122: Quantitative criteria for interstit
- Page 123 and 124: Quantitative criteria for fibrous i
- Page 125 and 126: C4d scoring :% biopsy area(cortex a
- Page 127 and 128: 2. Antibody-mediated changes:may co
- Page 129 and 130: 4. T-cell-mediated rejection (TCMR)
- Page 131 and 132: 6. Other:• changes not considered
- Page 133 and 134: References:• Banff 07 Classificat