PAS x40 - RCPA

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IP C3 <strong>x40</strong>Normal kidney - immunoperoxidasefor C3 with clean background, nostaining in the glomerulus andmoderate non-specific staining in theintima of arterioles and a small artery
IP C1q <strong>x40</strong>IP C1q x10Normal kidney - immunoperoxidasefor C1q with clean background andmoderate staining granular mesangialstaining, a non-specific finding inmany native and transplant renalbiopsies
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Laboratory handling of renal Bx:•
Page 7 and 8:
Our Medical Laboratory Scientist re
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Tissue for light microscopy:• des
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A paraffin embedded renal biopsy co
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Immunohistochemical stains:Native
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Examination of H&E stains:• on lo
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Medium power examination of H&E sta
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High power examination of H&E stain
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H&E x2This is an H&E stain of a ren
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H&E x10This is a higher power view.
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H&E x10This is renal medulla. Most
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marksglomerulimarks arteriolesmarks
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H&E x40marksglomeruli/Bowman’s ca
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H&E x40marks arteriolesoutlines par
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tubular polevascular pole
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peritubular capllariesarteriolessma
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H&E x2H&E x10This is a biopsy from
Page 39 and 40: atrophic tubules in anarea of inter
Page 41 and 42: segmental sclerosisfocal mild incre
Page 43 and 44: the mesangium is expandedby amorpho
Page 45 and 46: PAS x10
Page 47 and 48: Bowman’s capsulemesangiumtubular
Page 49 and 50: PAS x40Glomerulus sectioned through
Page 51 and 52: segmental sclerosisfocal hyalinosis
Page 53 and 54: OVG x40 - interlobulararteryOVG x40
Page 55 and 56: Masson x40 -normal glomerulusMasson
Page 57 and 58: Masson x40Masson x40variations in s
Page 59 and 60: Masson x40: glomerulus with mesangi
Page 61 and 62: AgMT x40 - small arteryAgMT x40 - g
Page 63 and 64: AgMT x100Can you identify mesangial
Page 65 and 66: This biopsy is from someonewith the
Page 67 and 68: AgMT x100 -variations in staining f
Page 69 and 70: mesangial cell nucleismallsubepithe
Page 71 and 72: AgMT x100:there is fuzzy materialor
Page 73 and 74: Congo red stain showing theapple gr
Page 75 and 76: Examination of immunohistochemical
Page 77 and 78: IF IgG x40Normal kidney -immunofluo
Page 79 and 80: IF fibrin x40Normal kidney -immunof
Page 81 and 82: IF C1q x40Normal kidney -immunofluo
Page 83 and 84: IP IgA x40Normal kidney - immunoper
Page 85 and 86: IP IgG x40Normal kidney - immunoper
Page 87 and 88: IP IgM x40Normal kidney - immunoper
Page 89: IP C3 x10
Page 93 and 94: IP kappa x40IP lambda x40Immunopero
Page 95 and 96: IP C4d x40Immunoperoxidase for C4d
Page 97 and 98: IP SV40 x40 biopsy BImmunoperoxidas
Page 99 and 100: Electron microscopy:• a piece of
Page 101 and 102: Toluidine blue x40Toluidine blue x2
Page 103 and 104: Assessment of the glomerulus in ele
Page 105 and 106: This is a low power electron microg
Page 107 and 108: This glomerulus is normocellular, w
Page 109 and 110: mesangial matrixepithelial cellfoot
Page 111 and 112: The Banff Classification of Renal A
Page 113 and 114: Specimen adequacy(a necessary prere
Page 115 and 116: Quantitative criteria for mononucle
Page 117 and 118: Quantitative criteria for arteriola
Page 119 and 120: Quantitative criteria for peritubul
Page 121 and 122: Quantitative criteria for interstit
Page 123 and 124: Quantitative criteria for fibrous i
Page 125 and 126: C4d scoring :% biopsy area(cortex a
Page 127 and 128: 2. Antibody-mediated changes:may co
Page 129 and 130: 4. T-cell-mediated rejection (TCMR)
Page 131 and 132: 6. Other:• changes not considered
Page 133 and 134: References:• Banff 07 Classificat
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PAS x40 - RCPA

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