PAS x40 - RCPA

Laboratory handling of renal Bx:• most operators provide cores 5 to 20mm long• two passes with a 14 gauge needle may be required to obtainadequate material, especially if other investigations arerequested; an 18 gauge needle may not provide an adequatespecimen of a native or transplanted kidney• cortex is required for both native and transplant assessment• medulla is also useful in transplant Bxs• in our hospital a specially trained Medical LaboratoryScientist attends all procedures except post reperfusiontransplant Bxs, and collects the specimen, looks for glomeruliusing a dissecting microscope and divides the tissue for– light microscopy– immunohistochemistry– electron microscopy– other investigations as requested