PAS x40 - RCPA

High power examination of H&E stains:• glomeruli– check thickness of sections by trying to focus up and downo if the section is too thick, there will be a range of planes– compare the diameter of the tuft with Bowman’s space– scant hyaline (bland glassy material) at the vascular pole is normalin older adults; hyaline elsewhere is abnormal– examine mesangial areaso with 0.5μ thick sections, there should be only 2 nuclei in any area;exclude the vascular pole from the cellularity assessmento matrix should be delicate, little more than the width of a mesangial cello capillary loops should be very delicate, but if obliquely sectioned, mayappear thicker than they are– endothelial cells are inconspicuous– capillary lumina should have only a few leucocytes(≤4 neutrophils/tuft) and should be patent– visceral epithelial cells should be easy to identify but not prominent;protein droplets are abnormal– parietal epithelial cells should be inconspicuous