MALDI Q-Tof Premier Operators Guide - Waters

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$Waters 2414 Refractive Index Detector$

Pattern editor ............................................................................................ 1-16Starting an acquisition .................................................................................. 1-17Initial instrument settings ....................................................................... 1-17Acquiring data from the Tune window .................................................... 1-202 Calibration ............................................................................................... 2-1Processing MALDI data ................................................................................... 2-2Calibrating .......................................................................................................... 2-4Using the Calibration window ........................................................................ 2-4Calibrating from previously processed data................................................... 2-5Using LockMass for Greater Mass Accuracy .............................................. 2-8Creating LockMass Correction........................................................................ 2-9Applying LockMass Correction ..................................................................... 2-10Internal LockMass .................................................................................... 2-10External LockMass from the Tune window ............................................. 2-10External LockMass from the Sample List ............................................... 2-103 MALDI Acquisition ................................................................................ 3-1Method Editor .................................................................................................... 3-2Sample List Formats ....................................................................................... 3-2Scan Conditions page....................................................................................... 3-2Scan Rate frame .......................................................................................... 3-2Instrument Mode frame .............................................................................. 3-3Source Settings frame ................................................................................. 3-3LockMass page................................................................................................. 3-5Peak detection ................................................................................................... 3-6MS Scan and MS Survey ............................................................................ 3-6MSMS Acquisition ...................................................................................... 3-9Software Setup ................................................................................................ 3-11MALDI MS method........................................................................................ 3-11MALDI MSMS method.................................................................................. 3-13MS/MS page ............................................................................................... 3-13Precursor Mass page ................................................................................. 3-14xTable of Contents
MALDI Survey method .................................................................................. 3-15MS to MSMS page ......................................................................................... 3-16Peaks for MS/MS ....................................................................................... 3-16Peak Selection from MS Survey Data ...................................................... 3-16EDC for MALDI methods ............................................................................... 3-17MS method ..................................................................................................... 3-17MSMS method................................................................................................ 3-17Survey method ............................................................................................... 3-17EDC calibration ............................................................................................. 3-17Auxiliary file output ....................................................................................... 3-18MS output files............................................................................................... 3-18MSMS output files ......................................................................................... 3-18Survey output files......................................................................................... 3-194 Maintenance and Troubleshooting .................................................... 4-1Cleaning the hexapole ..................................................................................... 4-2Cleaning and replacing the vacuum lock O-ring ...................................... 4-3Plate carrier errors .......................................................................................... 4-4Support for alternative sample plates ......................................................... 4-5Contacting Waters ............................................................................................ 4-65 Sample Preparation .............................................................................. 5-1Safety ................................................................................................................... 5-2Calibration standards ...................................................................................... 5-2Washing stainless steel MALDI plates ......................................................... 5-2Sample Preparation Considerations ............................................................ 5-3Matrices ............................................................................................................... 5-3Matrices and Substrates.................................................................................. 5-3Matrix Mixtures............................................................................................... 5-4Preparing Matrices ......................................................................................... 5-4Premix Method ............................................................................................ 5-5Table of Contentsxi
Page 1 and 2: Waters MicromassMALDI Q-Tof Premier
Page 3 and 4: Safety informationGeneralThe Waters
Page 5 and 6: Output specification of enclosed la
Page 7 and 8: MALDI Q-Tof Premier Spectrometer In
Page 9: Table of ContentsSafety information
Page 13 and 14: Glycolipids........................
Page 15 and 16: 1 Basic PrinciplesContents:TopicPag
Page 17 and 18: Installing the MALDI sourceBefore i
Page 19 and 20: Catch5. Press the Raise button and
Page 21 and 22: 6. Remove the Adaptor plate and Ape
Page 23 and 24: • Set parameters on the MALDI sou
Page 25 and 26: Load/Unload button states:StateBlue
Page 27 and 28: To open a user defined plate defini
Page 29 and 30: To align the laser position to the
Page 31 and 32: Spiral pattern in Pattern Editor:St
Page 33 and 34: Result: The MALDI control window op
Page 35 and 36: • What type of acquisition will b
Page 37 and 38: Result: The Sample List begins acqu
Page 39 and 40: 2 CalibrationCalibration for MALDI
Page 41 and 42: 8. In the Spectrum Center dialog bo
Page 43 and 44: With this box you can:• Choose an
Page 45 and 46: 8. Click File > Save As to save the
Page 47 and 48: Creating LockMass CorrectionTo crea
Page 49 and 50: To apply a LockMass correction:1. D
Page 51 and 52: 3 MALDI AcquisitionThe principles o
Page 53 and 54: The automatic scan rate is 5 second
Page 55 and 56: LockMass pageWhen ‘Acquire from t
Page 57 and 58: Peak monitor windowPeaks not consid
Page 59 and 60: This reduced list is then ranked in
Page 61 and 62:
MSMS Monitor window:Monitor window%
Page 63 and 64:
These are the same as those describ
Page 65 and 66:
• File in Sample List - select th
Page 67 and 68:
EDC for MALDI methodsMS methodEDC e
Page 69 and 70:
• _total.pklSurvey output filesAs
Page 71 and 72:
4 Maintenance andTroubleshootingIn
Page 73 and 74:
Cleaning and replacing the vacuum l
Page 75 and 76:
Support for alternative sample plat
Page 77 and 78:
5 Sample PreparationSample preparat
Page 79 and 80:
5. Dry under a dry nitrogen stream.
Page 81 and 82:
A washing stage or re-crystallizati
Page 83 and 84:
S-DHB (‘Super’ DHB)Add 1 mg of
Page 85 and 86:
Use the above concentrations as a s
Page 87 and 88:
8. Centrifuge and remove supernatan
Page 89 and 90:
9. Incubate the sample at 37 °C fo
Page 91 and 92:
Oligonucleotide CalibrationUse olig
Page 93 and 94:
this fundamental limitation of MALD
Page 95 and 96:
Structures of MALDI MatricesStructu
Page 97 and 98:
Structure and [M+H] + of Common MAL
Page 99 and 100:
IndexSymbols*.mtp 1-13*.ptn 1-16Num
Page 101 and 102:
peak detection 3-6MSMS Acquisition
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