MALDI Q-Tof Premier Operators Guide - Waters

Thin Film Technique ................................................................................... 5-5CHCA (a-cyano-4-hydroxy Cinnamic Acid) ............................................... 5-5Sinapinic Acid (3,5-dimethoxy-4-hydroxycinnamic Acid) ......................... 5-6Thin Film Sinapinic Acid ............................................................................ 5-6DHB (2,5-dihydroxybenzoic Acid) .............................................................. 5-6S-DHB (‘Super’ DHB) .................................................................................. 5-7CMBT (5-chloro-2-mercaptobenzothiazole) ............................................... 5-7Dithranol (1,8-Dihydroxy-9(10H)-anthracenone) ...................................... 5-7HABA (2-(4-hydroxyphenylazo)-benzoic Acid) ........................................... 5-7HPA (Hydroxypicolinic Acid) ...................................................................... 5-7IAA (b-indole acrylic Acid) .......................................................................... 5-7THAP (2,4,6-trihydroxyacetophenone) ...................................................... 5-7Anthranilic acid / Nicotinic Acid ................................................................ 5-84-hydroxy-a-phenylcinnamic Acid .............................................................. 5-83-aminoquinoline ......................................................................................... 5-8All Trans-retinoic Acid ................................................................................ 5-8Analysis of Peptides and Proteins ................................................................ 5-8Preparing Peptide and Protein Standards ..................................................... 5-9Preparing protein digests .............................................................................. 5-10In-gel protein digests ................................................................................ 5-10In-Solution Protein Digests ...................................................................... 5-11Internal LockMass correction from trypsin autolysis peptides................... 5-13Analysis of other compounds ....................................................................... 5-13Phosphopeptides ............................................................................................ 5-13Oligonucleotides............................................................................................. 5-14HPA Preparation with Desalting ............................................................. 5-14Oligonucleotide Calibration ...................................................................... 5-15Acquiring Data .......................................................................................... 5-15Oligosaccharides and Sugars ........................................................................ 5-15Sample Preparation .................................................................................. 5-15Oligosaccharide Calibration ..................................................................... 5-15Acquiring Data .......................................................................................... 5-15Glycoproteins ................................................................................................. 5-15xiiTable of Contents