MALDI Q-Tof Premier Operators Guide - Waters

More documents

Recommendations

Info

$Waters 2414 Refractive Index Detector$

5. Enter the sample well number in the sample box.The selected sample well is aligned with the laser beam.After the sample plate is loaded, the Unload button is highlighted blueto show that Unload is available.Neither button is highlighted when the instrument is acquiring data.To unload a plate:1. From the Tune window click .2. When the carrier has stopped moving open the plate carrier lid andremove the plate, taking care not to touch the surface.Plate carrier errorsThe current status of the plate carrier is displayed in the Tune window Statusbar. Error messages are displayed in the Plate Carrier Status Message dialogbox. If you connect the Heater/Interlock cable when a plate is already loaded,it is normal to receive the Status Message as shown.This message box indicates that the sample plate control is restarting. If theplate carrier is in the Unload position this message will not appear.Other errors give possible solutions in the dialog box and are indicated by thestatus of the Load/Unload buttons (see Plate carrier errors on page 4-4).1-10 Basic Principles
Load/Unload button states:StateBlueRedBoth disabledMeaningNormal operation - Load/UnloadRecoverable error. The plate is out of position.The error is cleared by clicking the buttons and theplate reindexes. If it does not recover contact Waters.This can be one of three things:• Acquiring data• Vented error• Plate carrier error (see page 4-4)Sample plate formatsThe MALDI Q-Tof Premier comes with several plate formats already defined.These are the standard 96 well MALDI sample plate (shown below), 384 well,MassPrep, DIOS and BigSpot.Standard 96 well sample plate:Sample Well A1LockMass well forA1, A2, B1, B2Viewing and setting plate formatsFrom the Tune window click Maldi > Sample Plate Definition to open theSample Plate Definition dialog box.Sample plates 1-11
Page 1 and 2: Waters MicromassMALDI Q-Tof Premier
Page 3 and 4: Safety informationGeneralThe Waters
Page 5 and 6: Output specification of enclosed la
Page 7 and 8: MALDI Q-Tof Premier Spectrometer In
Page 9 and 10: Table of ContentsSafety information
Page 11 and 12: MALDI Survey method ...............
Page 13 and 14: Glycolipids........................
Page 15 and 16: 1 Basic PrinciplesContents:TopicPag
Page 17 and 18: Installing the MALDI sourceBefore i
Page 19 and 20: Catch5. Press the Raise button and
Page 21 and 22: 6. Remove the Adaptor plate and Ape
Page 23: • Set parameters on the MALDI sou
Page 27 and 28: To open a user defined plate defini
Page 29 and 30: To align the laser position to the
Page 31 and 32: Spiral pattern in Pattern Editor:St
Page 33 and 34: Result: The MALDI control window op
Page 35 and 36: • What type of acquisition will b
Page 37 and 38: Result: The Sample List begins acqu
Page 39 and 40: 2 CalibrationCalibration for MALDI
Page 41 and 42: 8. In the Spectrum Center dialog bo
Page 43 and 44: With this box you can:• Choose an
Page 45 and 46: 8. Click File > Save As to save the
Page 47 and 48: Creating LockMass CorrectionTo crea
Page 49 and 50: To apply a LockMass correction:1. D
Page 51 and 52: 3 MALDI AcquisitionThe principles o
Page 53 and 54: The automatic scan rate is 5 second
Page 55 and 56: LockMass pageWhen ‘Acquire from t
Page 57 and 58: Peak monitor windowPeaks not consid
Page 59 and 60: This reduced list is then ranked in
Page 61 and 62: MSMS Monitor window:Monitor window%
Page 63 and 64: These are the same as those describ
Page 65 and 66: • File in Sample List - select th
Page 67 and 68: EDC for MALDI methodsMS methodEDC e
Page 69 and 70: • _total.pklSurvey output filesAs
Page 71 and 72: 4 Maintenance andTroubleshootingIn
Page 73 and 74: Cleaning and replacing the vacuum l
Page 75 and 76:
Support for alternative sample plat
Page 77 and 78:
5 Sample PreparationSample preparat
Page 79 and 80:
5. Dry under a dry nitrogen stream.
Page 81 and 82:
A washing stage or re-crystallizati
Page 83 and 84:
S-DHB (‘Super’ DHB)Add 1 mg of
Page 85 and 86:
Use the above concentrations as a s
Page 87 and 88:
8. Centrifuge and remove supernatan
Page 89 and 90:
9. Incubate the sample at 37 °C fo
Page 91 and 92:
Oligonucleotide CalibrationUse olig
Page 93 and 94:
this fundamental limitation of MALD
Page 95 and 96:
Structures of MALDI MatricesStructu
Page 97 and 98:
Structure and [M+H] + of Common MAL
Page 99 and 100:
IndexSymbols*.mtp 1-13*.ptn 1-16Num
Page 101 and 102:
peak detection 3-6MSMS Acquisition
show all

MALDI Q-Tof Premier Operators Guide - Waters

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?