Fluorescence

FLIPA part of a cell at a distance from where there is an excess of fluorescentprotein is bleached with a laser at low intensity. One then images the samplejust before and at constant intervals after bleaching, allowing the bleachedmolecules to redistribute through the cell – the loss of fluorescence isrecalculated to measure the diffusion dynamics.Applications: e.g.: checking the continuity of membrane systems (ER, Golgi).