- Page 1 and 2: Praktikum zur Fluoreszenz- undKonfo
- Page 4 and 5: Types of Light• Monochromatic•
- Page 6 and 7: Lenses, Focus and AberrationsReason
- Page 8 and 9: Types of microscopes andIlluminatio
- Page 10 and 11: ObjectivesDipping objectives-physio
- Page 14 and 15: Resolution!!!!!Magnification identi
- Page 16 and 17: Counts for transmitted and reflecte
- Page 18 and 19: Depth of FieldThe axial range, thro
- Page 20 and 21: Detection Systems Overviewhttp://bi
- Page 22 and 23: DetectionDigital Cameras: Photons e
- Page 24 and 25: Imaging - Triangle of Frustration©
- Page 26 and 27: FluorescenceStokes (1852) - Jablons
- Page 28 and 29: Principle of Fluorescence• Molecu
- Page 30 and 31: Quantum Efficiency• Only emitted
- Page 33: Fluorescent Dyes• Commercially av
- Page 36 and 37: Organelle Lights ORGANELLESMitochon
- Page 38 and 39: Quantum Dots“Whatever hue want”
- Page 40 and 41: IMMUNOFLUORESCENCE-MICROSCOPYIndire
- Page 42 and 43: FIXIERUNG:variable-should be suited
- Page 44 and 45: PERMEABILISIATION:For antibody diff
- Page 46 and 47: • ProLong is good for Alexa dyes
- Page 48 and 49: FLUORESCENCE MICROSCOPY
- Page 50 and 51: Light Sources / 2Laser• Light amp
- Page 52 and 53: Longpassfiltere.g. Longpass 420:Num
- Page 54 and 55: Bandpassfiltere.g.: Bandpass 465/70
- Page 56 and 57: Full Cube assembly Bandpass31001 (C
- Page 58 and 59: Problem 2: cross emission (emission
- Page 60 and 61: Pseudo-Confocal Microscopy - Struct
- Page 62 and 63:
Different grids - Differentsectioni
- Page 64 and 65:
Drawbacks - Troubleshooting• Phot
- Page 66 and 67:
DeconvolutionLimitations to the res
- Page 68 and 69:
Literature• J.Pawley, Handbook of
- Page 70:
Confocal Microscopywhat is differen
- Page 73 and 74:
Confocal: Functional ElementsDetect
- Page 75 and 76:
The Pinhole I• Light from below-f
- Page 77 and 78:
The Pinhole IIIA pinhole of 1 airy
- Page 79 and 80:
Confocal Overview II• A point lig
- Page 81 and 82:
Laser II - How are they used?• La
- Page 83 and 84:
Area Scanning - Point by Point
- Page 85 and 86:
Optics I: Overview
- Page 87 and 88:
Optics II: Selecting wavelengthsFil
- Page 89 and 90:
Confocal Overview IIIno interferenc
- Page 91 and 92:
Configurations - Zeiss LSM510-71028
- Page 93 and 94:
Live „Confocal“ - Many Pinholes
- Page 95 and 96:
Confocal Spinning DiscBeams 1Illumi
- Page 97 and 98:
Multiphoton MicroscopyPulsed lasers
- Page 99 and 100:
What can you do?Z- stack including
- Page 101 and 102:
Z-stack: Reducing Dimensions
- Page 103 and 104:
Z-stack: Reconstruction• X/Y/Z im
- Page 105 and 106:
Multicolor Labeling
- Page 107 and 108:
Problem II: cross- emissionWhen emi
- Page 109 and 110:
The Problems: How to they look like
- Page 111 and 112:
Spectral Imaging
- Page 113 and 114:
F-techniques - methodology
- Page 115 and 116:
iFRAP
- Page 117 and 118:
“Optical Highlighters”PHOTOACTI
- Page 119 and 120:
Photoactivation (and yet another F-
- Page 121 and 122:
Superresolution Ii.e. breaking the
- Page 123 and 124:
Superresolution using Fitting Algor