Fluorescence

Pseudo-confocal Microscopy-„Apotome“• Based on an interferometric principle of „gridprojection“ – Structured Illumination Microscopy• Getting rid of blurring –> depth of field informationonly• Grid with a defined width inserted into the plane ofthe field stop into the reflected light beam path ->grid lies in focal plane• Apotome scanner permits the grid to be shifted inposition upper and below focal plane• 3 images generated and superimposed – algorithmbased „deblurring“ -> pseudoconfocal image