Fluorescence

Depth of FieldThe axial range, through which an objective can be focused without any appreciablechange in image sharpness, is referred to as the objective depth of field = thickness alongthe z-axis where an object in the specimen appears focused !! -> almost only dependenton NA !Depth of Focus = the thickness of the image plane itself. Largely dependent onMagnification !MagnificationNumericalApertureDepthofField(mm)ImageDepth(mm)4x 0.10 15.5 0.1310x 0.25 8.5 0.8020x 0.40 5.8 3.840x 0.65 1.0 12.860x 0.85 0.40 29.8100x 0.95 0.19 80.0Large depth of FieldShallowdepth ofFieldhttp://www.olympusmicro.com/primer/anatomy/objectives.html