Fluorescence

DeconvolutionLimitations to the resolution in an optical system stems from „convolution“: glare,distortion and blurriness from stray light from out-of-focus areas, especially in fluorescencemicroscopy cause acquisition „artefacts“. Also in confocal microscopy these artefacts mayoccur from optical inconsistencies in the specimen, glass, or from optical defects (inpropercorrections) in objectives.Highly sophisticated software calculations can be applied to „reverse“ these artefacts andcreate crispy images for better evaluation.Why do we do it:•Enhanced resolution in all 3 dimensions x, y, and z•Reduction of Noise to improve S/N ratio•Reversal or optimization of optics-based aberrations