Fluorescence

Confocal Overview II• A point light source for illumination• A point light focus within the specimen• A pinhole at the image detecting plane• These three points are opticallyconjugated together and alignedaccurately to each other in the light pathof image formation, this is confocal.• Confocal effects result in supression ofout-of-focal-plane light, supression ofstray light in the final image