8453 UV-visible system - operator manual.pdf

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Installation and Start UpInstallation Summary for Your Agilent 8453 General Purpose UV-visible SystemPC❏❏❏❏❏❏Make sure that all of your PC components are connected to line power.Make sure that your general purpose software for UV-visible spectroscopy isinstalled.A printer must be configured on your PC.• Adjust paper size (for example, Letter, A4)• Set Orientation to PortraitThe TCP/IP protocol must be installed and configured on your PC.Your spectrophotometer must be configured with its IP address.If you connected your spectrophotometer directly to your PC be sure thatyour CAG Bootp Server application is installed and automatically launchedwhen you start your PC. If you connect to your spectrophotometer using aLAN make sure that your network administrator assigns the IP address to yourspectrophotometer.38
Installation and Start UpStarting a Measurement SessionStarting a Measurement SessionWith a network connection to your spectrophotometer it is important thatyour spectrophotometer is recognized by the software. This requires theassignment of a unique IP address at power on time to yourspectrophotometer. The assignment is either done by the CAG Bootp Serverapplication running on your PC with a direct connection to thespectrophotometer or in a LAN by a server application on your LAN.Therefore it is important that either one of these applications is up andrunning before you switch your spectrophotometer on.❏❏❏❏❏❏Switch on your PC and boot your PC operating system. If a printer isconnected to your system, switch the printer on.Make sure that the CAG Bootp Server is running or you are logged onto yourLAN.Switch your spectrophotometer on and wait until the spectrophotometer’sindicator light turns to green. This process includes the spectrophotometer’sself test and takes about one minute. For details on the startup sequence seechapter “Installation and Start Up” in your Installing Your UV-visibleSpectroscopy System manual.Launch your measurement session by pressing your operating system’s Startbutton and select Programs, UV-Visible ChemStations, spectrophotometer 1online.You are ready to use your system, if the blue busy status display on thesystem’s bottom message line turns off.The first measurement you have to perform is a reference measurement. Afterthis alignment your are ready to measure absorbance data and spectra.NOTEIt takes about 15 minutes for the lamps to reach stable state conditions. Forbest results, do not perform measurements before this period of time haselapsed.39
Page 1 and 2: Agilent 8453 UV-visibleSpectroscopy
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Page 5 and 6: Contents1 Introduction to Your Syst
Page 7 and 8: 11 Introduction to YourSystemConcep
Page 9 and 10: Introduction to Your System9
Page 11 and 12: Introduction to Your SystemOptical
Page 13 and 14: Introduction to Your SystemOptical
Page 15 and 16: Introduction to Your SystemSpectrop
Page 17 and 18: Introduction to Your SystemSpectrop
Page 19 and 20: Introduction to Your SystemUser Int
Page 21 and 22: Introduction to Your SystemUser Int
Page 23 and 24: Introduction to Your SystemSoftware
Page 26: Introduction to Your SystemStandard
Page 29 and 30: Introduction to Your SystemStandard
Page 35 and 36: 22 Installation and Start UpSummary
Page 37: Installation and Start UpInstallati
Page 41 and 42: 33 Good MeasurementPracticesGeneral
Page 43 and 44: Good Measurement PracticesGeneral C
Page 53 and 54: Good Measurement PracticesInserting
Page 55 and 56: 44 Using your Agilent 8453UV-visibl
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Using your Agilent 8453 UV-visible
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IndexNumerics2,2,4-trimethylpentane
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Indexn-hexane, 48nominal spectral s
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s1In This BookTo be able to use you
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